Alkaline Phosphatase Antibody (A-10): sc-271431

Alkaline Phosphatase Antibody (A-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects Alkaline Phosphatase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Alkaline Phosphatase antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Alkaline phosphatases (AP) are crucial enzymes that play a significant role in dephosphorylation processes, which are vital for various physiological functions, including bone mineralization and cellular signaling. Placental alkaline phosphatase (PLAP), a tissue-specific isoform, is particularly important as PLAP is expressed in the placenta and found in the serum of pregnant women, serving as a potential biomarker for certain cancers, including ovarian and testicular tumors. PLAP involvement in cellular migration and transport of essential molecules, such as fatty acids and immunoglobulins, across the plasma membrane highlights PLAP importance in both reproductive biology and cancer research. The three tissue-specific alkaline phosphatases identified in humans—PLAP, germ cell alkaline phosphatase (GCAP), and intestinal alkaline phosphatase—exhibit 90-98% homology and are clustered on chromosome 2q, indicating a shared evolutionary origin and functional significance in various biological contexts.

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Alkaline Phosphatase Antibody (A-10) References:

1. Profile of placental alkaline phosphatase expression in human malignancies: effect of tumour cell activation on alkaline phosphatase expression.  |  Dabare, AA., et al. 1999. Urol Int. 63: 168-74. PMID: 10738188
2. Crystal structure of alkaline phosphatase from human placenta at 1.8 A resolution. Implication for a substrate specificity.  |  Le Du, MH., et al. 2001. J Biol Chem. 276: 9158-65. PMID: 11124260
3. Placental-type alkaline phosphatase in peritoneal fluid of women with endometriosis.  |  Kang, JO., et al. 1990. Clin Chim Acta. 186: 285-94. PMID: 2311256
4. Placental alkaline phosphatase-like isoenzymes produced by human gastric cancer cells.  |  Aizawa, K., et al. 1989. Acta Pathol Jpn. 39: 630-7. PMID: 2686348
5. Serum placental-like alkaline phosphatase (PLAP): a novel combined enzyme linked immunoassay for monitoring ovarian cancer.  |  Fisken, J., et al. 1989. J Clin Pathol. 42: 40-5. PMID: 2921344
6. Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.  |  Tucker, DF., et al. 1985. Br J Cancer. 51: 631-9. PMID: 2986664
7. Diagnostic aspects of alkaline phosphatase and its isoenzymes.  |  Moss, DW. 1987. Clin Biochem. 20: 225-30. PMID: 3319285
8. Human placental and intestinal alkaline phosphatase genes map to 2q34-q37.  |  Griffin, CA., et al. 1987. Am J Hum Genet. 41: 1025-34. PMID: 3687940
9. Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours.  |  Epenetos, AA., et al. 1985. Br J Cancer. 51: 641-4. PMID: 3994908
10. Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.  |  Travers, P. and Bodmer, W. 1984. Int J Cancer. 33: 633-41. PMID: 6202646
11. An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.  |  Epenetos, AA., et al. 1984. Br J Cancer. 49: 11-5. PMID: 6362705
12. Biology of human alkaline phosphatases with special reference to cancer.  |  Millán, JL. and Fishman, WH. 1995. Crit Rev Clin Lab Sci. 32: 1-39. PMID: 7748466

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Alkaline Phosphatase Antibody (A-10) has 44 citations in a variety of scientific publications.

Primary antibodies like Anti-Alkaline Phosphatase Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Alkaline Phosphatase Antibody (A-10) Specifications

Alternate Product Name: Alkaline Phosphatase

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 66-111

Epitope Target: PLAP

Epitope Species: human

Product Reactivity/Detection: PLAP, ALPPL2 and IAP of human origin, and Akp-3 of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 44 citations in various scientific publications

Available Conjugates/Forms for Alkaline Phosphatase Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred TNAP antibody is TNAP Antibody (F-4): sc-166261.