Alix Antibody (G-10): sc-166952

Alix Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Alix of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Alix antibody (G-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The ALG-2-interacting protein, commonly known as Alix, plays a crucial role in cellular processes by facilitating the sorting and concentration of cargo proteins within multivesicular bodies, which are essential for proper intracellular transport and communication. Alix is primarily located in the cytoplasm, where Alix interacts with several key proteins, including apoptosis-associated proteins such as ALG-2 and PDCD6, as well as endocytosis-regulating proteins like CIN85. Additionally, Alix is involved in recruiting endosomal sorting complexes required for transport (ESCRT) proteins, such as Tsg101 and CHMP4, which are vital for multivesicular body formation and HIV-1 budding regulation. Alix binding with endophilins, enzymes that modify membrane curvature, underscores Alix importance in early and late stages of coated vesicle formation. This multifaceted role in cellular dynamics highlights Alix significance in both normal physiological processes and disease states, making Alix monoclonal antibody (G-10) an invaluable research tool for studying these critical pathways.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

Download thiomersal SDS

Alix Antibody (G-10) References:

1. Extracellular Alix regulates integrin-mediated cell adhesions and extracellular matrix assembly.  |  Pan, S., et al. 2008. EMBO J. 27: 2077-90. PMID: 18636094
2. Exploring the functional interaction between POSH and ALIX and the relevance to HIV-1 release.  |  Votteler, J., et al. 2009. BMC Biochem. 10: 12. PMID: 19393081
3. Natural deletion of L35Y36 in p6 gag eliminate LYPXnL/ALIX auxiliary virus release pathway in HIV-1 subtype C.  |  Patil, A. and Bhattacharya, J. 2012. Virus Res. 170: 154-8. PMID: 22981647
4. Centrosomal ALIX regulates mitotic spindle orientation by modulating astral microtubule dynamics.  |  Malerød, L., et al. 2018. EMBO J. 37: PMID: 29858227
5. CCL2 mobilizes ALIX to facilitate Gag-p6 mediated HIV-1 virion release.  |  Ajasin, DO., et al. 2019. Elife. 8: PMID: 31172941
6. The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis.  |  Addi, C., et al. 2020. Nat Commun. 11: 1941. PMID: 32321914
7. Abrogating ALIX Interactions Results in Stuttering of the ESCRT Machinery.  |  Gupta, S., et al. 2020. Viruses. 12: PMID: 32948012
8. ALIX promotes cell migration and invasion of head and neck squamous cell carcinoma by regulating the expression of MMP9, MMP14, VEGF-C.  |  Xie, QH., et al. 2023. Arch Oral Biol. 151: 105696. PMID: 37086494
9. Increased Expression of Tetraspanins, ALIX and HSP-70 in the Placenta Tissue of Women with Chronic Venous Disease during Pregnancy.  |  Ortega, MA., et al. 2023. Int J Med Sci. 20: 1744-1754. PMID: 37928882
10. NEDD4 family ubiquitin ligase AIP4 interacts with Alix to enable HBV naked capsid egress in an Alix ubiquitination-independent manner.  |  Shen, S., et al. 2024. PLoS Pathog. 20: e1012485. PMID: 39259704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Alix Antibody (G-10) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Alix Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Alix Antibody (G-10) Specifications

Alternate Product Name: Alix

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 463-732

Epitope Target: Alix

Epitope Species: human

Product Reactivity/Detection: Alix

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Alix Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Alix antibody is Alix Antibody (1A12): sc-53540.