Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aldolase A CRISPR/Cas9 KO Plasmid (m): sc-419082
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aldolase A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aldolase A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aldolase A Antibody (C-10): sc-390733
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aldolase A CRISPR/Cas9 KO Plasmid (m) | sc-419082 | 20 µg | $397.00 |
Overview
Aldoa encodes aldolase A, a glycolytic enzyme that catalyzes the reversible cleavage of fructose-1,6-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, linking glucose utilization to ATP production and biosynthetic precursor generation. In mouse cells, aldolase A supports metabolic flux through glycolysis and interfaces with broader carbon metabolism programs that influence redox balance and cellular proliferation. Beyond metabolism, aldolase A has been implicated in cytoskeletal organization through interactions with actin dynamics, connecting energy production with cell shape and motility. Altered ALDOA expression and glycolytic rewiring are frequently studied in contexts of metabolic stress and disease-associated phenotypes, including tumor-associated metabolic adaptation and muscle-related functional deficits.
Aldolase A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aldoa gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aldoa together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aldoa open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aldolase A protein expression.
This CRISPR knockout system enables efficient generation of Aldoa-deficient cell models for investigation of Aldolase A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aldoa exon(s) critical for Aldolase A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aldoa genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aldolase A CRISPR/Cas9 KO Plasmid (m) and Aldolase A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aldoa locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aldolase A HDR Plasmid (m) and Aldolase A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aldoa homology arms to support homology-directed repair at defined Aldoa target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.