Akt1 Antibody (F-8): sc-271149

Akt1 Antibody (F-8) is a mouse monoclonal IgG1 antibody that detects Akt1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Akt1, also known as protein kinase B (PKB), plays a crucial role in cellular signaling pathways that regulate cell growth, survival, and metabolism, making Akt1 a key player in cancer biology and metabolic disorders. Akt1 protein is primarily located in the cytoplasm and can translocate to the plasma membrane upon activation, which is essential for mediating signals from growth factors such as insulin and platelet-derived growth factor (PDGF). This translocation enables Akt1 to interact with phosphatidylinositol 3-kinase (PI3K) and other signaling molecules, facilitating downstream signaling that promotes cell survival and proliferation. Furthermore, Akt1 undergoes post-translational modifications, including phosphorylation at specific residues, which are vital for activation and function. The phosphorylation of Thr 308 and Ser 473 is particularly important, as these modifications enhance Akt1′s enzymatic activity and ability to regulate various cellular processes. Anti-Akt1 antibody (F-8) serves as an invaluable tool for researchers studying the intricate signaling pathways involving Akt1 and its implications in health and disease.

The preferred Akt1 antibody is Akt1 Antibody (B-1): sc-5298.