Akp-5 CRISPR/Cas9 KO Plasmid (m): sc-419070
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Akp-5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Akp-5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Akp-5 CRISPR/Cas9 KO Plasmid (m) | sc-419070 | 20 µg | $397.00 |
Overview
Alppl2 encodes the mouse alkaline phosphatase isoform Akp-5, a glycosylphosphatidylinositol (GPI)-anchored ectoenzyme that dephosphorylates extracellular nucleotides and other phosphomonoesters at the cell surface. Through regulation of local phosphate availability and purinergic signaling intermediates, Akp-5 can influence membrane-associated metabolic processes, epithelial differentiation programs, and microenvironmental cues that affect cell–cell interactions. Alppl2 expression has been reported in contexts linked to developmental state and lineage identity, making it useful as a marker and functional node in studies of differentiation and tissue remodeling. Dysregulated alkaline phosphatase activity is frequently examined in relation to altered cellular maturation and stress responses, supporting investigation of Alppl2 in models of abnormal growth control and disease-relevant phenotypes.
Akp-5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Alppl2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Alppl2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Alppl2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Akp-5 protein expression.
This CRISPR knockout system enables efficient generation of Alppl2-deficient cell models for investigation of Akp-5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Alppl2 exon(s) critical for Akp-5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Alppl2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Akp-5 CRISPR/Cas9 KO Plasmid (m) and Akp-5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Alppl2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Akp-5 HDR Plasmid (m) and Akp-5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Alppl2 homology arms to support homology-directed repair at defined Alppl2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.