AKAP 9 Double Nickase Plasmid (h2): sc-405430-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AKAP 9 Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AKAP 9 Double Nickase Plasmid (h2) and AKAP 9 Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting AKAP9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AKAP 9 Antibody (7E12): sc-517030
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AKAP 9 Double Nickase Plasmid (h2) | sc-405430-NIC-2 | 20 µg | $410.00 |
Human AKAP9 (A-kinase anchoring protein 9) is a large scaffold protein that spatially organizes PKA and additional signaling enzymes at the Golgi apparatus, centrosome, and microtubule-organizing centers to coordinate cAMP-dependent signaling with cytoskeletal dynamics. It contributes to microtubule nucleation and spindle assembly, supports Golgi integrity, and helps regulate cell-cycle progression and cell polarity through compartmentalized kinase/phosphatase activities. AKAP9 dysregulation and structural alterations have been linked to genome instability and oncogenic rearrangements, and it is also studied in contexts of cardiac excitability and neurodevelopmental phenotypes where signaling microdomains are perturbed. Gene editing of AKAP9 enables mechanistic interrogation of subcellular signaling scaffolds, centrosome and Golgi biology, and genotype–phenotype relationships in models of proliferative and excitable tissues.
AKAP 9 Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the AKAP9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AKAP9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AKAP9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AKAP9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.