AKAP 7 Double Nickase Plasmid (h): sc-406308-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AKAP 7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AKAP 7 Double Nickase Plasmid (h) and AKAP 7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AKAP7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AKAP 7 Antibody (1F9): sc-517021
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AKAP 7 Double Nickase Plasmid (h) | sc-406308-NIC | 20 µg | $410.00 | |||
AKAP 7 Double Nickase Plasmid (h2) | sc-406308-NIC-2 | 20 µg | $410.00 |
AKAP7 encodes A-kinase anchoring protein 7, a scaffold that spatially constrains cAMP-dependent protein kinase A (PKA) signaling by tethering PKA to discrete subcellular compartments. By organizing local signaling microdomains, AKAP7 contributes to the timing and specificity of phosphorylation events that influence ion channel regulation, calcium handling, and broader second-messenger pathway crosstalk. This compartmentalization links AKAP7 to processes such as excitation–contraction coupling, signal-dependent membrane dynamics, and phosphorylation-controlled transcriptional responses. Dysregulated AKAP–PKA anchoring has been associated with altered cellular signaling states relevant to cardiometabolic and neurophysiological phenotypes, supporting its use as a target for mechanistic studies of signaling fidelity.
AKAP 7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AKAP7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AKAP7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AKAP7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AKAP7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.