AKAP 7 Antibody (1F9): sc-517021
- AKAP 7 Antibody (1F9) is a mouse monoclonal IgG2b κ AKAP 7 antibody provided at 100 µg/ml
- raised against amino acids 2-115 representing partial length AKAP 7 of human origin
- recommended for detection of AKAP 7 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for AKAP 7 Antibody (1F9). This work is in progress.
AKAP 7 Antibody (1F9) is a mouse monoclonal IgG2b kappa light chain antibody that detects AKAP 7 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-AKAP 7 antibody (1F9) is available as a non-conjugated format. AKAP 7, also known as AKAP18, is a critical component in regulating protein kinase A (PKA) signaling pathways, which are essential for various cellular processes, including gene transcription, cell growth, and metabolism. This 104 amino acid protein is predominantly expressed in brain, heart, lung, pancreas, and skeletal muscle, where AKAP 7 plays a vital role in anchoring PKA to plasma membrane, facilitating interaction with L-type calcium channels. Precise PKA localization is crucial for function, ensuring kinase activation in proper cellular context and allowing specific signaling events to occur. AKAP 7 exists in four isoforms due to alternative splicing, with γ isoform being particularly significant as AKAP 7 binds to regulatory subunit RI of PKA, potentially influencing PKA-mediated gene transcription in both somatic cells and oocytes. AKAP 7′s ability to compartmentalize PKA signaling underscores importance in maintaining cellular homeostasis and responding to physiological stimuli.
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AKAP 7 Antibody (1F9) References:
- Coordination of cAMP signaling events through PKA anchoring. | Scott, JD., et al. 2000. Adv Pharmacol. 47: 175-207. PMID: 10582087
- Alternative splicing regulates the subcellular localization of A-kinase anchoring protein 18 isoforms. | Trotter, KW., et al. 1999. J Cell Biol. 147: 1481-92. PMID: 10613906
- A-kinase anchoring proteins: protein kinase A and beyond. | Edwards, AS. and Scott, JD. 2000. Curr Opin Cell Biol. 12: 217-21. PMID: 10712918
- Role and identification of protein kinase A anchoring proteins in vasopressin-mediated aquaporin-2 translocation. | Klussmann, E. and Rosenthal, W. 2001. Kidney Int. 60: 446-9. PMID: 11473624
- AKAP7gamma is a nuclear RI-binding AKAP. | Brown, RL., et al. 2003. Biochem Biophys Res Commun. 306: 394-401. PMID: 12804576
- Identification of a novel A-kinase anchoring protein 18 isoform and evidence for its role in the vasopressin-induced aquaporin-2 shuttle in renal principal cells. | Henn, V., et al. 2004. J Biol Chem. 279: 26654-65. PMID: 15037626
- High-affinity AKAP7delta-protein kinase A interaction yields novel protein kinase A-anchoring disruptor peptides. | Hundsrucker, C., et al. 2006. Biochem J. 396: 297-306. PMID: 16483255
- Spatial organisation of AKAP18 and PDE4 isoforms in renal collecting duct principal cells. | McSorley, T., et al. 2006. Eur J Cell Biol. 85: 673-8. PMID: 16500722
- AKAP18 contains a phosphoesterase domain that binds AMP. | Gold, MG., et al. 2008. J Mol Biol. 375: 1329-43. PMID: 18082768
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AKAP 7 Antibody (1F9) | sc-517021 | 100 µg/ml | $322.00 |