AKAP 10 Antibody (C-8) recommended for detection of AKAP 10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

AKAP 10 Antibody (C-8): sc-271074

AKAP 10 Antibody (C-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-AKAP 10 Antibody (C-8) is a mouse monoclonal IgG1 (kappa light chain) AKAP 10 antibody provided at 200 µg/ml
    • raised against amino acids 508-662 mapping at the C-terminus of AKAP 10 of human origin
    • Anti-AKAP 10 Antibody (C-8) is recommended for detection of AKAP 10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-AKAP 10 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AKAP 10 (C-8): sc-271074.
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Can AKAP 10 (C-8): sc-271074 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, AKAP 10 (C-8): sc-271074 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by from WB: clean bands in human AKAP 10 transfected WB: clean bands in human AKAP 10 transfected lysates. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Works well for western blotting Works well for western blotting - tested in RAW 264.7, NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from Positive results in western blot analysis Positive results in western blot analysis of AKAP 10 expression in mouse brain tissue extract. -SCBT QC
Date published: 2015-02-06
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