Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ajuba Double Nickase Plasmid (h): sc-403298-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ajuba Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ajuba Double Nickase Plasmid (h) and Ajuba Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AJUBA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ajuba Antibody (B-3): sc-398008
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ajuba Double Nickase Plasmid (h) | sc-403298-NIC | 20 µg | $410.00 |
AJUBA encodes Ajuba, a LIM domain–containing scaffold protein that localizes to adherens junctions and the nucleus, where it integrates signals controlling cell adhesion, cytoskeletal dynamics, and mechanotransduction. Ajuba participates in pathways linked to Hippo/YAP signaling, MAPK/ERK signaling, and regulation of transcriptional programs through interactions with multiple kinases and transcriptional regulators. Through these roles, AJUBA helps coordinate contact inhibition, epithelial organization, and stress-responsive gene expression. Dysregulated AJUBA expression or function has been associated with altered migratory behavior and proliferation programs observed in cancer and other disorders involving barrier dysfunction and aberrant tissue remodeling.
Ajuba Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AJUBA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AJUBA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AJUBA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AJUBA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.