AIM1 CRISPR/Cas9 KO Plasmid (h): sc-410680
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AIM1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AIM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AIM1 CRISPR/Cas9 KO Plasmid (h) | sc-410680 | 20 µg | $397.00 |
Overview
AIM1 (absent in melanoma 1) encodes a cytoskeletal-associated protein implicated in organizing actin-rich structures and maintaining cellular architecture. It has been linked to regulation of cell shape, adhesion, and migratory behavior through effects on cytoskeletal dynamics and related signaling networks. Altered AIM1 expression or genomic disruption has been reported across multiple tumor types, supporting its use as a research target in studies of transformation, invasion, and metastatic phenotypes. AIM1 is also studied in the context of lineage differentiation and tissue organization where cytoskeletal remodeling is central to cell state transitions.
AIM1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AIM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AIM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AIM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AIM1 protein expression.
This CRISPR knockout system enables efficient generation of AIM1-deficient cell models for investigation of AIM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AIM1 exon(s) critical for AIM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AIM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AIM1 CRISPR/Cas9 KO Plasmid (h) and AIM1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AIM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AIM1 HDR Plasmid (h) and AIM1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AIM1 homology arms to support homology-directed repair at defined AIM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.