AIF Antibody (E-11): sc-390619

AIF Antibody (E-11) is a mouse monoclonal IgM kappa light chain antibody specific for an epitope mapping between amino acids 586-612 at the C-terminus of Apoptosis-Inducing Factor (AIF) of mouse origin. AIF monoclonal antibody (E-11) detects AIF from mouse, rat, and human origins by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA); AIF monoclonal antibody (E-11) is also reactive with additional species including equine, canine, bovine, and porcine. AIF, also known as AIFM1 or PDCD8 (Programmed Cell Death Protein 8), is a flavoprotein located in the mitochondrial intermembrane space, characterized by FAD- and NADH-binding domains that contribute to oxidoreductase activity. AIF′s structure facilitates a dual role in mitochondrial energy metabolism and apoptosis; upon apoptotic stimuli, AIF translocates from mitochondria to nucleus where AIF induces chromatin condensation and large-scale DNA fragmentation, leading to programmed cell death. Understanding AIF′s structure and localization is important because dysregulation of AIF function has been implicated in various pathological conditions including neurodegenerative diseases and cancer. Anti-AIF antibody (E-11) is a valuable tool for researchers studying mechanisms of apoptosis and mitochondrial biology, providing high specificity and affinity for reliable detection of AIF across multiple species and experimental applications. AIF monoclonal antibody (E-11) aids in advancing research into cell death pathways and their implications in human health and disease by targeting the C-terminal region.

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AIF Antibody (E-11) References:

1. Apoptosis-inducing factor: vital and lethal.  |  Modjtahedi, N., et al. 2006. Trends Cell Biol. 16: 264-72. PMID: 16621561
2. Apoptosis-inducing factor and colon cancer.  |  Millan, A. and Huerta, S. 2009. J Surg Res. 151: 163-70. PMID: 18061616
3. Apoptosis-inducing factor: structure, function, and redox regulation.  |  Sevrioukova, IF. 2011. Antioxid Redox Signal. 14: 2545-79. PMID: 20868295
4. Expression of apoptosis-inducing factor (AIF) in keratoacanthomas and squamous cell carcinomas of the skin.  |  Skyrlas, A., et al. 2011. Exp Dermatol. 20: 674-6. PMID: 21410769
5. Apoptosis-inducing factor (AIF) is targeted in IFN-α2a-induced Bid-mediated apoptosis through Bak activation in ovarian cancer cells.  |  Miyake, K., et al. 2012. Biochim Biophys Acta. 1823: 1378-88. PMID: 22683989
6. Apoptosis-inducing factor (AIF) nuclear translocation mediated caspase-independent mechanism involves in X-ray-induced MCF-7 cell death.  |  Yang, S., et al. 2017. Int J Radiat Biol. 93: 270-278. PMID: 27809636
7. The apoptosis-inducing factor family: Moonlighting proteins in the crosstalk between mitochondria and nuclei.  |  Novo, N., et al. 2021. IUBMB Life. 73: 568-581. PMID: 33035389
8. Apoptosis-inducing factor plays a role in the pathogenesis of hepatic and renal injury during cholestasis.  |  Ghanbarinejad, V., et al. 2021. Naunyn Schmiedebergs Arch Pharmacol. 394: 1191-1203. PMID: 33527194
9. Apoptosis-inducing factor-like protein-mediated stress and metronidazole-responsive programmed cell death pathway in Entamoeba histolytica.  |  Bandyopadhyay, A. and Ghosh, SK. 2023. Mol Microbiol. 119: 640-658. PMID: 37037799
10. Allosteric Inhibitors of Macrophage Migration Inhibitory Factor (MIF) Interfere with Apoptosis-Inducing Factor (AIF) Co-Localization to Prevent Parthanatos.  |  Chen, D., et al. 2023. J Med Chem. 66: 8767-8781. PMID: 37352470

The preferred AIF antibody is AIF Antibody (E-1): sc-13116.