AID Antibody (ZA001): sc-517548

AID Antibody (ZA001) is a mouse monoclonal IgG1 kappa light chain antibody that detects AID protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-AID antibody (ZA001) is available as the non-conjugated format. Activation-induced Cytidine Deaminase (AID, HIGM-2) is a 198-amino acid RNA-editing enzyme that plays a crucial role in B-cell terminal differentiation, specifically in immunoglobulin class switch recombination and somatic hypermutation. AID is predominantly expressed in germinal center B cells, where AID facilitates the generation of high-affinity antibodies, including IgG, IgA, and IgE, which are essential for effective immune responses. Absence of AID in patients with Hyper-IgM syndrome (HIGM2) leads to significant impairment in these processes, resulting in lack of immunoglobulin class switching and somatic hypermutations, as well as lymph node hyperplasia characterized by enlarged germinal centers filled with active B cells. Furthermore, AID-/- mice exhibit a hyper-IgM phenotype, underscoring AID′s importance in multiple stages of B-cell differentiation necessary for robust antibody production. AID monoclonal antibody (ZA001) accurately detects AID across various applications, making this reagent invaluable for researchers studying B-cell biology and related immunological disorders.

The preferred AID antibody is AID Antibody (H-3): sc-518170.