Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
AGRP CRISPR/Cas9 KO Plasmid (m): sc-419045
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AGRP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AGRP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AGRP CRISPR/Cas9 KO Plasmid (m) | sc-419045 | 20 µg | $397.00 |
Overview
Agouti-related neuropeptide (AGRP), encoded by the mouse Agrp gene, is an orexigenic neuropeptide predominantly expressed in arcuate nucleus neurons that regulate energy balance and feeding behavior. AGRP acts as an endogenous antagonist or inverse agonist at melanocortin receptors, especially MC3R and MC4R, countering anorexigenic melanocortin signaling to modulate appetite, adiposity, and metabolic rate. Agrp-expressing neurons integrate endocrine and nutrient cues and interface with hypothalamic circuits controlling glucose homeostasis and neuroendocrine outputs. Dysregulation of AGRP-linked pathways is frequently studied in models of obesity, metabolic syndrome, diabetes-associated phenotypes, and stress-related alterations in ingestive behavior.
AGRP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Agrp gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Agrp together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Agrp open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AGRP protein expression.
This CRISPR knockout system enables efficient generation of Agrp-deficient cell models for investigation of AGRP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Agrp exon(s) critical for AGRP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Agrp genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AGRP CRISPR/Cas9 KO Plasmid (m) and AGRP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Agrp locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AGRP HDR Plasmid (m) and AGRP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Agrp homology arms to support homology-directed repair at defined Agrp target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.