Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
AGR2 Double Nickase Plasmid (m): sc-423838-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AGR2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AGR2 Double Nickase Plasmid (m) and AGR2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Agr2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AGR2 Antibody (6C5): sc-101211
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AGR2 Double Nickase Plasmid (m) | sc-423838-NIC | 20 µg | $410.00 |
Mouse Agr2 encodes anterior gradient 2 (AGR2), an endoplasmic reticulum (ER)–resident protein disulfide isomerase–like factor that supports oxidative protein folding and secretory pathway homeostasis. AGR2 contributes to ER proteostasis programs, including unfolded protein response signaling and quality control of client proteins destined for secretion or membrane localization. Through these functions, AGR2 influences epithelial differentiation, mucin processing, and cell–cell interaction dynamics in tissues with high secretory demand. Dysregulated AGR2 expression has been linked to stress adaptation and altered signaling in models of epithelial dysfunction and tumor biology, making Agr2 a useful node for probing ER stress, proteostasis, and secretory network regulation.
AGR2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Agr2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Agr2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Agr2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Agr2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.