AGPS Antibody (A-2): sc-374201
- AGPS Antibody (A-2) is a mouse monoclonal IgG1 κ AGPS antibody, cited in 9 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 577-609 near the C-terminus of AGPS of human origin
- AGPS Antibody (A-2) is recommended for detection of AGPS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-AGPS Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for AGPS Antibody (A-2) for WB and IHC(P) applications. This reagent is now offered in a bundle with AGPS Antibody (A-2) (see ordering information below).
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AGPS Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects AGPS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AGPS (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AGPS protein, also known as alkyldihydroxyacetonephosphate synthase or aging-associated gene 5 protein, is a crucial enzyme consisting of 658 amino acids that plays a vital role in glycerolipid metabolism and ether lipid biosynthesis. Located on the inner aspect of the peroxisomal membrane, AGPS functions as part of a heterotrimeric complex alongside GNPAT and a modified form of GNPAT, which is essential for the synthesis of alkyl-glycerone 3-phosphate and long-chain acid anions from 1-acetyl-glycerone 3-phosphate and long-chain alcohols. A FAD-binding PCMH-type domain allows AGPS to utilize flavin adenine dinucleotide as a cofactor, underscoring its importance in metabolic pathways. Deficiencies in the gene encoding AGPS can lead to rhizomelic chondrodysplasia punctata type 3, a severe genetic disorder characterized by vertebral abnormalities, significant cognitive impairment, skin lesions, cataracts, and shortening of the humerus and femur.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AGPS Antibody (A-2) References:
- Alkyl-dihydroxyacetone phosphate synthase and dihydroxyacetone phosphate acyltransferase form a protein complex in peroxisomes. | Biermann, J., et al. 1999. Eur J Biochem. 261: 492-9. PMID: 10215861
- Impaired membrane traffic in defective ether lipid biosynthesis. | Thai, TP., et al. 2001. Hum Mol Genet. 10: 127-36. PMID: 11152660
- Alkyl-dihydroxyacetonephosphate synthase. | de Vet, EC. and van den Bosch, H. 2000. Cell Biochem Biophys. 32 Spring: 117-21. PMID: 11330037
- Isolation and characterization of mutant animal cell line defective in alkyl-dihydroxyacetonephosphate synthase: localization and transport of plasmalogens to post-Golgi compartments. | Honsho, M., et al. 2008. Biochim Biophys Acta. 1783: 1857-65. PMID: 18571506
- Lysine acetylation targets protein complexes and co-regulates major cellular functions. | Choudhary, C., et al. 2009. Science. 325: 834-40. PMID: 19608861
- Peroxisome dependency of alkyl-containing GPI-anchor biosynthesis in the endoplasmic reticulum. | Kanzawa, N., et al. 2009. Proc Natl Acad Sci U S A. 106: 17711-6. PMID: 19815513
- Nucleotide sequence of human alkyl-dihydroxyacetonephosphate synthase cDNA reveals the presence of a peroxisomal targeting signal 2. | de Vet, EC., et al. 1997. Biochim Biophys Acta. 1346: 25-9. PMID: 9187299
- Alkyl-dihydroxyacetonephosphate synthase. Fate in peroxisome biogenesis disorders and identification of the point mutation underlying a single enzyme deficiency. | de Vet, EC., et al. 1998. J Biol Chem. 273: 10296-301. PMID: 9553082
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AGPS Antibody (A-2) | sc-374201 | 200 µg/ml | $322.00 | |||
AGPS Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-552023 | 200 µg Ab; 10 µg BP | $361.00 | |||
AGPS Antibody (A-2) AC | sc-374201 AC | 500 µg/ml, 25% agarose | $424.00 | |||
AGPS Antibody (A-2) HRP | sc-374201 HRP | 200 µg/ml | $322.00 | |||
AGPS Antibody (A-2) FITC | sc-374201 FITC | 200 µg/ml | $336.00 | |||
AGPS Antibody (A-2) PE | sc-374201 PE | 200 µg/ml | $349.00 | |||
AGPS Antibody (A-2) Alexa Fluor® 488 | sc-374201 AF488 | 200 µg/ml | $364.00 | |||
AGPS Antibody (A-2) Alexa Fluor® 546 | sc-374201 AF546 | 200 µg/ml | $364.00 | |||
AGPS Antibody (A-2) Alexa Fluor® 594 | sc-374201 AF594 | 200 µg/ml | $364.00 | |||
AGPS Antibody (A-2) Alexa Fluor® 647 | sc-374201 AF647 | 200 µg/ml | $364.00 | |||
AGPS Antibody (A-2) Alexa Fluor® 680 | sc-374201 AF680 | 200 µg/ml | $364.00 | |||
AGPS Antibody (A-2) Alexa Fluor® 790 | sc-374201 AF790 | 200 µg/ml | $364.00 | |||
AGPS (A-2) Neutralizing Peptide | sc-374201 P | 100 µg/0.5 ml | $69.00 |