Agmatinase Double Nickase Plasmid (h): sc-405176-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Agmatinase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Agmatinase Double Nickase Plasmid (h) and Agmatinase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AGMAT. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Agmatinase Antibody (G-12): sc-166414
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Agmatinase Double Nickase Plasmid (h) | sc-405176-NIC | 20 µg | $410.00 | |||
Agmatinase Double Nickase Plasmid (h2) | sc-405176-NIC-2 | 20 µg | $410.00 |
AGMAT encodes agmatinase, a manganese-dependent metalloenzyme that hydrolyzes agmatine to putrescine and urea, linking arginine-derived agmatine metabolism to the polyamine biosynthetic network. Through regulation of putrescine availability, agmatinase influences downstream spermidine and spermine pools that support nucleic acid packaging, translational control, and cell-cycle progression. AGMAT activity intersects with nitric oxide–related arginine flux and cellular stress responses by modulating agmatine, a bioactive amine with neuromodulatory and metabolic signaling roles. Dysregulated polyamine homeostasis and altered agmatine/arginine metabolism have been associated with cancer biology, neurobiology, and inflammatory phenotypes, making AGMAT a useful target for mechanistic studies of metabolic rewiring.
Agmatinase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AGMAT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AGMAT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AGMAT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AGMAT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.