AFP/Alpha fetoprotein Antibody (C3): sc-8399

AFP Antibody (C3) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect Alpha-fetoprotein (AFP), also known as Alpha 1 Fetoprotein or Alpha-fetoglobulin, across multiple species including mouse, rat, and human. Anti-AFP antibody (C3) targets the full-length AFP protein, recognizing amino acids 1-609, and is effective in various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). AFP plays a crucial role during fetal development, primarily expressed in the liver, and is typically present in minimal amounts in adult tissues. The significance of AFP lies in its elevated levels in hepatocellular carcinoma and other liver-related cancers, making AFP a vital tumor marker for early detection and monitoring of cancer progression. Additionally, AFP has the ability to bind essential metals like copper and nickel, as well as fatty acids, and may interact with serum albumin or estrogen, highlighting its multifunctional nature. The regulation of AFP expression involves key transcription factors such as NF-1, HNF-1, and C/EBP, where HNF-1 promotes AFP expression and NF-1 inhibits its promoter activity. AFP monoclonal antibody (C3) is available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing flexibility for diverse experimental needs. Anti-AFP antibody (C3) is an essential tool for researchers studying liver development, cancer biology, and protein interactions involving AFP, ensuring accurate and reliable results in their investigations.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

AFP/Alpha fetoprotein Antibody (C3) References:

1. Effect of alpha-fetoprotein and albumin on the uptake of polyunsaturated fatty acids by rat hepatoma cells and fetal rat hepatocytes.  |  Iturralde, M., et al. 1991. Biochim Biophys Acta. 1086: 81-8. PMID: 1720022
2. Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes.  |  Stefanová, I., et al. 1988. J Immunol Methods. 111: 67-73. PMID: 2455753
3. Members of the CAAT/enhancer-binding protein, hepatocyte nuclear factor-1 and nuclear factor-1 families can differentially modulate the activities of the rat alpha-fetoprotein promoter and enhancer.  |  Bois-Joyeux, B. and Danan, JL. 1994. Biochem J. 301 (Pt 1): 49-55. PMID: 7518671
4. Gene therapy for hepatoma cells using a retrovirus vector carrying herpes simplex virus thymidine kinase gene under the control of human alpha-fetoprotein gene promoter.  |  Ido, A., et al. 1995. Cancer Res. 55: 3105-9. PMID: 7541712
5. [Several transcription factors participate in the functioning of the alpha-fetoprotein gene promoter].  |  Bois-Joyeux, B., et al. 1995. Bull Cancer. 82: 541-50. PMID: 7549116
6. Copper(II)-binding ability of human alpha-fetoprotein.  |  Aoyagi, Y., et al. 1978. Cancer Res. 38: 3483-6. PMID: 80265
7. Stimulation of tumor-cell growth by alpha-fetoprotein.  |  Wang, XW. and Xu, B. 1998. Int J Cancer. 75: 596-9. PMID: 9466662
8. Flavonoids inhibit estrogen binding to rat alpha-fetoprotein.  |  Baker, ME., et al. 1998. Proc Soc Exp Biol Med. 217: 317-21. PMID: 9492341

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AFP/Alpha fetoprotein Antibody (C3) has 88 citations in a variety of scientific publications.

Primary antibodies like Anti-AFP/Alpha fetoprotein Antibody (C3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AFP/Alpha fetoprotein Antibody (C3) Specifications

Alternate Product Name: AFP

Product Clone Name: C3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-609

Epitope Target: AFP

Epitope Species: human

Product Reactivity/Detection: AFP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 88 citations in various scientific publications

Available Conjugates/Forms for AFP/Alpha fetoprotein Antibody (C3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin