AFAP-1L2 Antibody (12): sc-136176

AFAP-1L2 Antibody (12) is a mouse monoclonal IgG2a kappa light chain antibody that detects AFAP-1L2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-AFAP-1L2 antibody (12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AFAP-1L2, also known as XB130, is a cytoplasmic protein consisting of 818 amino acids and features two Pleckstrin homology (PH) domains, which are critical for its role in intracellular signaling. AFAP-1L2 is primarily expressed in the thyroid and spleen, with lower expression levels found in the lung, brain, pancreas, and kidney. AFAP-1L2 is involved in the regulation of actin organization and interacts with Src kinases, suggesting a potential role in Src-regulated transcription activation. Four isoforms of AFAP-1L2, generated through alternative splicing, add complexity to its functional repertoire, allowing for diverse regulatory mechanisms in cellular signaling pathways. By linking signaling molecules to actin filaments, AFAP-1L2 provides a scaffold for the assembly of larger signaling complexes, thereby influencing various cellular processes.

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AFAP-1L2 Antibody (12) References:

1. The DNA sequence and comparative analysis of human chromosome 10.  |  Deloukas, P., et al. 2004. Nature. 429: 375-81. PMID: 15164054
2. Protein kinase Calpha activates c-Src and induces podosome formation via AFAP-110.  |  Gatesman, A., et al. 2004. Mol Cell Biol. 24: 7578-97. PMID: 15314167
3. Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules.  |  Zhang, Y., et al. 2005. Mol Cell Proteomics. 4: 1240-50. PMID: 15951569
4. XB130, a novel adaptor protein for signal transduction.  |  Xu, J., et al. 2007. J Biol Chem. 282: 16401-12. PMID: 17412687
5. Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.  |  Rikova, K., et al. 2007. Cell. 131: 1190-203. PMID: 18083107
6. Odin (ANKS1A) is a Src family kinase target in colorectal cancer cells.  |  Emaduddin, M., et al. 2008. Cell Commun Signal. 6: 7. PMID: 18844995
7. XB130, a tissue-specific adaptor protein that couples the RET/PTC oncogenic kinase to PI 3-kinase pathway.  |  Lodyga, M., et al. 2009. Oncogene. 28: 937-49. PMID: 19060924
8. An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells.  |  Heibeck, TH., et al. 2009. J Proteome Res. 8: 3852-61. PMID: 19534553

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AFAP-1L2 Antibody (12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-AFAP-1L2 Antibody (12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AFAP-1L2 Antibody (12) Specifications

Alternate Product Name: AFAP-1L2

Product Clone Name: 12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: AFAP-1L2

Epitope Species: human

Product Reactivity/Detection: AFAP-1L2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for AFAP-1L2 Antibody (12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin