AFAP-1L1 Double Nickase Plasmid (h): sc-407503-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AFAP-1L1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AFAP-1L1 Double Nickase Plasmid (h) and AFAP-1L1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AFAP1L1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AFAP-1L1 Antibody (D-6): sc-514788
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AFAP-1L1 Double Nickase Plasmid (h) | sc-407503-NIC | 20 µg | $410.00 | |||
AFAP-1L1 Double Nickase Plasmid (h2) | sc-407503-NIC-2 | 20 µg | $410.00 |
AFAP1L1 encodes AFAP-1L1, an actin filament–associated adaptor protein that links signaling complexes to cytoskeletal remodeling. AFAP-1L1 participates in processes such as stress fiber organization, focal adhesion dynamics, and cell shape changes through interactions that couple Src-family kinase signaling to actin-regulatory machinery. By modulating adhesion and motility programs, AFAP1L1 is frequently studied in the context of invasive behavior, epithelial–mesenchymal transition–like phenotypes, and metastatic dissemination models. Altered AFAP1L1 expression or pathway engagement has been reported across multiple tumor types, supporting its utility as a node for mechanistic studies of migration and cytoskeletal signaling networks.
AFAP-1L1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AFAP1L1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AFAP1L1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AFAP1L1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AFAP1L1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.