AFAP-1L1 Antibody (D-6): sc-514788

AFAP-1L1 Antibody (D-6) is a mouse monoclonal IgM antibody that detects AFAP-1L1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AFAP-1L1 (D-6) antibody is available as the non-conjugated form. AFAP-1L1, also known as Actin filament-associated protein 1-like 1, plays a crucial role in cellular signaling and actin organization, consisting of 768 amino acids and featuring two Pleckstrin homology (PH) domains typically associated with proteins involved in intracellular signaling pathways. AFAP-1L1 is phosphorylated in response to DNA damage, likely by ATR or ATM kinases, suggesting a role in cellular stress response and genomic integrity maintenance. AFAP-1L1 interacts with various signaling molecules, serving as an adaptor that links these molecules to actin filaments, facilitating larger signaling complex formation and influencing actin dynamics. Four isoforms of AFAP-1L1, generated through alternative splicing, highlight regulatory potential in different cellular contexts.

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AFAP-1L1 Antibody (D-6) References:

1. The carboxy terminus of AFAP-110 modulates direct interactions with actin filaments and regulates its ability to alter actin filament integrity and induce lamellipodia formation.  |  Qian, Y., et al. 2000. Exp Cell Res. 255: 102-13. PMID: 10666339
2. The actin filament-associated protein AFAP-110 is an adaptor protein that modulates changes in actin filament integrity.  |  Baisden, JM., et al. 2001. Oncogene. 20: 6435-47. PMID: 11607843
3. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases.  |  Baisden, JM., et al. 2001. Oncogene. 20: 6607-16. PMID: 11641786
4. Molecular cloning of actin filament-associated protein: a putative adaptor in stretch-induced Src activation.  |  Lodyga, M., et al. 2002. Am J Physiol Lung Cell Mol Physiol. 283: L265-74. PMID: 12114187
5. Characterization of long cDNA clones from human adult spleen. II. The complete sequences of 81 cDNA clones.  |  Jikuya, H., et al. 2003. DNA Res. 10: 49-57. PMID: 12693554
6. Protein kinase Calpha activates c-Src and induces podosome formation via AFAP-110.  |  Gatesman, A., et al. 2004. Mol Cell Biol. 24: 7578-97. PMID: 15314167
7. Conversion of mechanical force into biochemical signaling.  |  Han, B., et al. 2004. J Biol Chem. 279: 54793-801. PMID: 15485829
8. The PH domain: a common piece in the structural patchwork of signalling proteins.  |  Musacchio, A., et al. 1993. Trends Biochem Sci. 18: 343-8. PMID: 8236453

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AFAP-1L1 Antibody (D-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-AFAP-1L1 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AFAP-1L1 Antibody (D-6) Specifications

Alternate Product Name: AFAP-1L1

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 57-74

Epitope Target: AFAP-1L1

Epitope Species: human

Product Reactivity/Detection: AFAP-1L1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for AFAP-1L1 Antibody (D-6): P: Blocking Peptide