Afamin Antibody (G-10): sc-373849

Afamin Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Afamin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Afamin antibody (G-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Afamin, also known as Alpha-albumin, is a 599 amino acid protein that belongs to the ALB/AFP/VDB family, which consists of structurally related serum transport proteins encoded by four genes located in a tandem arrangement on chromosome four. Afamin is primarily expressed in the liver and secreted into the bloodstream, where Afamin plays a crucial role in the transport of various ligands, although the specific ligand remains unidentified. Notably, Afamin contains three albumin domains and undergoes N-glycosylation, a post-translational modification that is essential for stability and function, influencing interaction with other proteins and overall biological activity. Afamin′s ability to bind and transport ligands effectively underscores potential importance in various physiological processes, including nutrient transport and regulation of blood plasma levels, making Afamin a significant target for research in metabolic and cardiovascular diseases.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Afamin Antibody (G-10) References:

1. Bilirubin binding properties of pigeon serum albumin and its comparison with human serum albumin.  |  Khan, MA., et al. 2002. Int J Biol Macromol. 30: 171-8. PMID: 12063119
2. Characterization of the vitamin E-binding properties of human plasma afamin.  |  Voegele, AF., et al. 2002. Biochemistry. 41: 14532-8. PMID: 12463752
3. Afamin is a novel human vitamin E-binding glycoprotein characterization and in vitro expression.  |  Jerkovic, L., et al. 2005. J Proteome Res. 4: 889-99. PMID: 15952736
4. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.  |  Liu, T., et al. 2005. J Proteome Res. 4: 2070-80. PMID: 16335952
5. Structural and functional mapping of alpha-fetoprotein.  |  Terentiev, AA. and Moldogazieva, NT. 2006. Biochemistry (Mosc). 71: 120-32. PMID: 16489915
6. Biological and clinical aspects of the vitamin D binding protein (Gc-globulin) and its polymorphism.  |  Speeckaert, M., et al. 2006. Clin Chim Acta. 372: 33-42. PMID: 16697362
7. Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.  |  Ramachandran, P., et al. 2006. J Proteome Res. 5: 1493-503. PMID: 16740002
8. Proteome analysis of human follicular fluid.  |  Angelucci, S., et al. 2006. Biochim Biophys Acta. 1764: 1775-85. PMID: 17067859
9. Afamin--A pleiotropic glycoprotein involved in various disease states.  |  Dieplinger, H. and Dieplinger, B. 2015. Clin Chim Acta. 446: 105-10. PMID: 25892677
10. Afamin expression in breast cancer.  |  Wang, WK., et al. 2020. Asian J Surg. 43: 750-754. PMID: 31653553
11. Afamin predicts the prevalence and incidence of nonalcoholic fatty liver disease.  |  Pitkänen, N., et al. 2022. Clin Chem Lab Med. 60: 243-251. PMID: 34850615
12. Serum afamin levels in predicting gestational diabetes mellitus and preeclampsia: A systematic review and meta-analysis.  |  Yuan, Y., et al. 2023. Front Endocrinol (Lausanne). 14: 1157114. PMID: 37033215

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Afamin Antibody (G-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Afamin Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Afamin Antibody (G-10) Specifications

Alternate Product Name: Afamin

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 454-507

Epitope Target: Afamin

Epitope Species: human

Product Reactivity/Detection: Afamin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Afamin Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin