AF-6 Antibody (G-7): sc-398370

AF-6 Antibody (G-7) is a mouse monoclonal IgM antibody that detects AF-6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AF-6, also known as MLLT4 (myeloid/lymphoid or mixed-lineage leukemia translocated to 4), is a crucial 1,612 amino acid protein that plays a significant role in the dynamics of cell-cell interfaces, particularly at tight junctions and adherens junctions. AF-6 contains two N-terminal Ras binding domains and a GLGF motif, which are essential for involvement in Ras-mediated signaling events at peripheral cell-cell junctions. AF-6 interacts with F-actin and Profilin, suggesting a role in modulating actin dynamics near adhesion complexes, which is vital for maintaining cellular architecture and integrity. Additionally, AF-6 coordinates the recruitment of junction adhesion molecules (JAM) to intercellular junctions via PDZ domain, highlighting importance in regulating cell adhesion and communication. Studies have shown that developing mice deficient in AF-6 exhibit a loss of neuroepithelial polarity, underscoring AF-6′s critical function in influencing apical/basolateral asymmetry and overall cellular organization. Anti-AF-6 antibody (G-7) is an invaluable tool for researchers investigating the mechanisms of cell adhesion and signaling pathways in various physiological and pathological contexts.

The preferred AF-6 antibody is AF-6 Antibody (B-5): sc-74433.