AF-6 Antibody (B-5) recommended for detection of AF-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

AF-6 Antibody (B-5): sc-74433

AF-6 Antibody (B-5) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-AF-6 Antibody (B-5) is a mouse monoclonal IgG2a (kappa light chain) AF-6 antibody provided at 200 µg/ml
    • raised against amino acids 5-110 of AF-6 of human origin
    • Anti-AF-6 Antibody (B-5) is recommended for detection of AF-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-AF-6 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AF-6 (B-5): sc-74433.

Can AF-6 (B-5): sc-74433 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, AF-6 (B-5): sc-74433 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Good antibody Works good with MDCk cells at 1:50 dillution for IF Also works for WB but at 1:200 dillution
Date published: 2018-01-31
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic staining Beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-05-10
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