AF-6 Antibody (B-5): sc-74433

AF-6 Antibody (B-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects AF-6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AF-6 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AF-6 protein, also known as MLLT4 (myeloid/lymphoid or mixed-lineage leukemia translocated to 4), is a crucial 1,612 amino acid protein that plays a significant role in cell-cell adhesion and signaling. AF-6 contains two N-terminal Ras binding domains and a GLGF motif, which are essential for interaction with Ras proteins at peripheral cell junctions. AF-6 interacts with F-actin and Profilin, thereby influencing actin dynamics at adhesion complexes, which is vital for maintaining structural integrity of tissues. AF-6 coordinates recruitment of junction adhesion molecules (JAMs) to intercellular junctions through PDZ domain, highlighting importance in regulating cell polarity and junction integrity. Studies have shown that developing mice lacking AF-6 exhibit loss of neuroepithelial polarity, underscoring AF-6′s critical role in establishing and maintaining apical/basolateral asymmetry in epithelial tissues.

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AF-6 Antibody (B-5) References:

1. Absence of the tight junctional protein AF-6 disrupts epithelial cell-cell junctions and cell polarity during mouse development.  |  Zhadanov, AB., et al. 1999. Curr Biol. 9: 880-8. PMID: 10469590
2. Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1.  |  Ebnet, K., et al. 2000. J Biol Chem. 275: 27979-88. PMID: 10856295
3. The junctional multidomain protein AF-6 is a binding partner of the Rap1A GTPase and associates with the actin cytoskeletal regulator profilin.  |  Boettner, B., et al. 2000. Proc Natl Acad Sci U S A. 97: 9064-9. PMID: 10922060
4. Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in acute myeloid leukemias with the t(6;11) chromosome translocation.  |  Prasad, R., et al. 1993. Cancer Res. 53: 5624-8. PMID: 8242616
5. Identification of AF-6 and canoe as putative targets for Ras.  |  Kuriyama, M., et al. 1996. J Biol Chem. 271: 607-10. PMID: 8557659
6. The Ras target AF-6 interacts with ZO-1 and serves as a peripheral component of tight junctions in epithelial cells.  |  Yamamoto, T., et al. 1997. J Cell Biol. 139: 785-95. PMID: 9348294

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AF-6 Antibody (B-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-AF-6 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AF-6 Antibody (B-5) Specifications

Alternate Product Name: AF-6

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 5-110

Epitope Target: AF-6

Epitope Species: human

Product Reactivity/Detection: AF-6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for AF-6 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin