AF-6 Antibody (C-3): sc-74432
- AF-6 Antibody (C-3) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 5-110 of AF-6 of human origin
- recommended for detection of AF-6 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG2a BP-HRP is the preferred secondary detection reagent for AF-6 Antibody (C-3) for WB applications. This reagent is now offered in a bundle with AF-6 Antibody (C-3) (see ordering information below).
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SEE ALSO...
AF-6 Antibody (C-3) is a mouse monoclonal IgG2a antibody that detects AF-6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AF-6, also known as MLLT4 (myeloid/lymphoid or mixed-lineage leukemia translocated to 4), is a crucial 1,612 amino acid protein that plays a significant role in regulating cell-cell junctions, which are vital for maintaining tissue integrity and cellular communication. AF-6 features two N-terminal Ras binding domains and a GLGF motif, which are essential for involvement in Ras-mediated signaling pathways at peripheral cell-cell junctions. AF-6 interacts with F-actin and Profilin, contributing to the modulation of actin dynamics near adhesion complexes, thereby influencing the structural organization of the cytoskeleton. AF-6 coordinates the recruitment of junction adhesion molecules (JAMs) to intercellular junctions through PDZ domain, highlighting AF-6′s importance in the assembly and maintenance of tight junctions. Studies have shown that developing mice deficient in AF-6 exhibit a loss of neuroepithelial polarity, underscoring AF-6′s critical role in regulating cell-cell junctions that affect apical/basolateral asymmetry. Anti-AF-6 antibody (C-3) is an invaluable tool for researchers investigating the mechanisms of cell adhesion and signaling in various physiological and pathological contexts.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AF-6 Antibody (C-3) | sc-74432 | 200 µg/ml | $322.00 | |||
AF-6 Antibody (C-3): m-IgG2a BP-HRP Bundle | sc-546011 | 200 µg Ab; 10 µg BP | $361.00 |