Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
AdSS1 CRISPR/Cas9 KO Plasmid (m): sc-419030
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AdSS1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AdSS1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AdSS1 Antibody (G-9): sc-166401
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AdSS1 CRISPR/Cas9 KO Plasmid (m) | sc-419030 | 20 µg | $397.00 |
Overview
Mouse Adssl1 encodes AdSS1, an adenylosuccinate synthase family enzyme that supports de novo purine biosynthesis by catalyzing adenylosuccinate formation, a key step in AMP production. Through its contribution to nucleotide homeostasis, AdSS1 helps sustain DNA/RNA synthesis, energy charge, and proliferative capacity, linking it to core metabolic programs that couple mitochondrial function and cellular growth. Perturbation of purine metabolism can disrupt replication and stress-response pathways and is frequently associated with metabolic dysfunction and altered cell cycle control. As a muscle-enriched paralog within the pathway, Adssl1 is relevant for studying tissue-specific regulation of nucleotide flux and its downstream impacts on bioenergetics and cellular physiology.
AdSS1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adssl1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adssl1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adssl1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AdSS1 protein expression.
This CRISPR knockout system enables efficient generation of Adssl1-deficient cell models for investigation of AdSS1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adssl1 exon(s) critical for AdSS1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adssl1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AdSS1 CRISPR/Cas9 KO Plasmid (m) and AdSS1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adssl1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AdSS1 HDR Plasmid (m) and AdSS1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adssl1 homology arms to support homology-directed repair at defined Adssl1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.