ADK Antibody (F-5): sc-365470

ADK Antibody (F-5) is a mouse monoclonal IgG1 antibody that detects adenosine kinase (ADK) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ADK plays a crucial role in cellular metabolism by catalyzing the transfer of the gamma-phosphate from ATP to adenosine, thereby regulating the levels of both extracellular adenosine and intracellular adenine nucleotides. This regulation is vital as adenosine acts as an important extracellular signaling molecule, influencing various physiological processes across the cardiovascular, nervous, respiratory, and immune systems, particularly during tissue injury and inflammation. The human ADK gene, located on chromosome 10q11-q24, produces two isoforms of the enzyme, differing in their N-terminal sequences, yet both maintain identical kinetic properties and require magnesium ions for their activity. Notably, ADK′s ability to form soluble aggregates at higher concentrations can lead to enzyme inactivation, which underscores the importance of maintaining optimal conditions for its function. Anti-ADK antibody (F-5) is an essential tool for researchers studying the intricate roles of adenosine in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ADK Antibody (F-5) References:

1. Aryl diketoacids (ADK) selectively inhibit duplex DNA-unwinding activity of SARS coronavirus NTPase/helicase.  |  Lee, C., et al. 2009. Bioorg Med Chem Lett. 19: 1636-8. PMID: 19233643
2. Uncoupling of astrogliosis from epileptogenesis in adenosine kinase (ADK) transgenic mice.  |  Li, T., et al. 2008. Neuron Glia Biol. 4: 91-9. PMID: 19674507
3. Adenosine Triggers Larval Settlement and Metamorphosis in the Mussel Mytilopsis sallei through the ADK-AMPK-FoxO Pathway.  |  He, J., et al. 2021. ACS Chem Biol. 16: 1390-1400. PMID: 34254778
4. NLRP3 Inflammasome Activation Enhances ADK Expression to Accelerate Epilepsy in Mice.  |  Zhang, H., et al. 2022. Neurochem Res. 47: 713-722. PMID: 34797502
5. Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.  |  Wölkart, G., et al. 2022. Eur J Pharmacol. 927: 175050. PMID: 35618039
6. ADK-VR2, a cell line derived from a treatment-naïve patient with SDC4-ROS1 fusion-positive primarily crizotinib-resistant NSCLC: a novel preclinical model for new drug development of ROS1-rearranged NSCLC.  |  Ruzzi, F., et al. 2022. Transl Lung Cancer Res. 11: 2216-2229. PMID: 36519016
7. Exosome-derived miR-182-5p promoted cholangiocarcinoma progression and vasculogenesis by regulating ADK/SEMA5a/PI3K pathway.  |  Wang, J., et al. 2024. Liver Int. 44: 370-388. PMID: 37950359
8. Expression of ADK-S and ADK-L Isoforms and Their Association with CD39/CD73/A2aR in Colorectal Cancer.  |  Zhulai, GA. and Shibaev, MI. 2023. Bull Exp Biol Med. 176: 91-95. PMID: 38085399
9. The Interaction between ADK and SCG10 Regulate the Repair of Nerve Damage.  |  Chen, T., et al. 2024. Neuroscience. 544: 75-87. PMID: 38423163
10. Hyperibone J exerts antidepressant effects by targeting ADK to inhibit microglial P2X7R/TLR4-mediated neuroinflammation.  |  Li, T., et al. 2024. J Adv Res.. PMID: 39019111

The preferred ADK antibody is ADK Antibody (H-1): sc-514588.