Adenylate cyclase 6/AC6/ADCY6 CRISPR/Cas9 KO Plasmid (m): sc-418996

Overview

Adcy6 encodes adenylate cyclase 6 (AC6/ADCY6), a membrane-associated enzyme that converts ATP to cAMP downstream of G protein–coupled receptor signaling. By shaping cAMP dynamics, AC6 regulates PKA- and EPAC-dependent phosphorylation programs that influence ion channel activity, metabolic control, and stimulus-dependent transcriptional responses. In mouse tissues, ADCY6 is commonly linked to β-adrenergic and other Gs-coupled pathways that coordinate cardiac contractility, airway smooth muscle tone, and renal salt/water handling. Dysregulated cAMP signaling involving ADCY6 has been associated with cardiopulmonary and kidney-relevant phenotypes, supporting its use in mechanistic studies of GPCR signal integration and second-messenger homeostasis.

Adenylate cyclase 6/AC6/ADCY6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adcy6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adcy6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adcy6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Adenylate cyclase 6/AC6/ADCY6 protein expression.

This CRISPR knockout system enables efficient generation of Adcy6-deficient cell models for investigation of Adenylate cyclase 6/AC6/ADCY6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Adcy6 exon(s) critical for Adenylate cyclase 6/AC6/ADCY6 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Adcy6 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Adenylate cyclase 6/AC6/ADCY6 CRISPR/Cas9 KO Plasmid (m) and Adenylate cyclase 6/AC6/ADCY6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adcy6 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Adenylate cyclase 6/AC6/ADCY6 HDR Plasmid (m) and Adenylate cyclase 6/AC6/ADCY6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adcy6 homology arms to support homology-directed repair at defined Adcy6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.