Adenosine A2A-R Antibody (7F6-G5-A2) recommended for detection of Adenosine A2A-R of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Adenosine A2A-R Antibody (7F6-G5-A2): sc-32261

Adenosine A2A-R Antibody (7F6-G5-A2) is rated 5.0 out of 5 by 8.
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Datasheets

    • Anti-Adenosine A2A-R Antibody (7F6-G5-A2) is a mouse monoclonal IgG2a (kappa light chain) Adenosine A2A-R antibody provided at 200 µg/ml
    • raised against His-tagged full length human recombinant Adenosine A2A receptor, epitope mapping to the third intracellular loop
    • Anti-Adenosine A2A-R Antibody (7F6-G5-A2) is recommended for detection of Adenosine A2A-R of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-Adenosine A2A-R Antibody (7F6-G5-A2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP or PerCP-Cy5.5 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Adenosine A2A-R (7F6-G5-A2): sc-32261.

Dear, which Ab dilution, temperature and incubation time do you recommend for the sc-32261 FITC staining on mouse cells for FACS analysis? Any specific protocols? I guess I need to fix and permeabilize since the epitope is intracellular.  Thanks

Asked by: CC84
Thank you for your question. You will find our recommended protocol in the following link : https://www.scbt.com/scbt/resources/protocols/flow-cytometry. If you require more information, please contact our Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

How can I use this mouse monoclonal primary antibody for use with mouse samples?

Asked by: Anonymous
The use of mouse-host antibodies on mouse samples is typically not problematic. To reduce nonspecific binding by a secondary reagent, a conjugated form of the primary antibody can be used. Adenosine A2A-R Antibody (7F6-G5-A2): sc-32261 is available conjugated to HRP (sc-32261 HRP), phycoerythrin (sc-32261 PE), fluoroscein (sc-32261 FITC), Alexa Fluor® 488 (sc-32261 AF488), Alexa Fluor® 647 (sc-32261 AF647), and PerCP (sc-32261 PerCP).
Answered by: Technical Support 8
Date published: 2017-01-04
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Rated 5 out of 5 by from Nice results Nice WB, IHC and FCM results produced with this antibody
Date published: 2017-01-19
Rated 5 out of 5 by from Superb Very clear western and ihc results;low background !
Date published: 2017-01-02
Rated 5 out of 5 by from Good publishable data in IHC in rat primary Good publishable data in IHC in rat primary neuronal cells. -SCBT Publication Review
Date published: 2015-04-06
Rated 5 out of 5 by from Good staining in IF in rabbit acinar cells Good staining in IF in rabbit acinar cells. -SCBT Publication Review
Date published: 2015-02-26
Rated 5 out of 5 by from Produced beautiful immunoperoxidase membrane Produced beautiful immunoperoxidase membrane staining in formalin fixed, paraffin-embedded mouse brain tissue. -SCBT QC
Date published: 2015-02-25
Rated 5 out of 5 by from Positive signal in WB in human transfected Positive signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-10
Rated 5 out of 5 by from Robust signal in WB in mouse brain tissue extract Robust signal in WB in mouse brain tissue extract. -SCBT QC
Date published: 2015-01-02
Rated 5 out of 5 by from Produced great FCM data for human peripheral Produced great FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2014-05-16
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