ADAT1 Antibody (C-5): sc-271812
- ADAT1 Antibody (C-5) is a mouse monoclonal IgG1 κ ADAT1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 13-37 near the N-terminus of ADAT1 of mouse origin
- ADAT1 Antibody (C-5) is recommended for detection of ADAT1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ADAT1 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for ADAT1 Antibody (C-5) for WB applications. This reagent is now offered in a bundle with ADAT1 Antibody (C-5) (see ordering information below).
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ADAT1 Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADAT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ADAT1 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADAT1 plays a crucial role in RNA editing, specifically in the modification of tRNA molecules, where ADAT1 catalyzes the conversion of adenosine to inosine in tRNAAla transcripts. This modification is essential for proper tRNA functioning, influencing protein translation accuracy and gene expression fidelity. ADAT1′s ability to facilitate these modifications underscores ADAT1′s importance in cellular processes, particularly in stress responses and gene expression regulation. Additionally, ADAT1 belongs to a larger family of double-stranded RNA adenosine deaminases, including ADAR1 and ADAR2, that participate in RNA editing, highlighting the interconnectedness of these proteins in maintaining RNA integrity and function. ADAT1′s presence and interactions with other RNA-binding proteins are vital for dynamic gene expression regulation, making ADAT1 a significant target for research in molecular biology and genetics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ADAT1 Antibody (C-5) References:
- Regulation of alternative splicing by RNA editing. | Rueter, SM., et al. 1999. Nature. 399: 75-80. PMID: 10331393
- Editing of messenger RNA precursors and of tRNAs by adenosine to inosine conversion. | Keller, W., et al. 1999. FEBS Lett. 452: 71-6. PMID: 10376681
- Identification and characterization of a human tRNA-specific adenosine deaminase related to the ADAR family of pre-mRNA editing enzymes. | Maas, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 8895-900. PMID: 10430867
- The importance of internal loops within RNA substrates of ADAR1. | Lehmann, KA. and Bass, BL. 1999. J Mol Biol. 291: 1-13. PMID: 10438602
- The solution structure of the Zalpha domain of the human RNA editing enzyme ADAR1 reveals a prepositioned binding surface for Z-DNA. | Schade, M., et al. 1999. Proc Natl Acad Sci U S A. 96: 12465-70. PMID: 10535945
- Structural requirements for RNA editing in glutamate receptor pre-mRNAs by recombinant double-stranded RNA adenosine deaminase. | Maas, S., et al. 1996. J Biol Chem. 271: 12221-6. PMID: 8647818
- RED2, a brain-specific member of the RNA-specific adenosine deaminase family. | Melcher, T., et al. 1996. J Biol Chem. 271: 31795-8. PMID: 8943218
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAT1 Antibody (C-5) | sc-271812 | 200 µg/ml | $322.00 | |||
ADAT1 Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-531693 | 200 µg Ab; 20 µg BP | $361.00 | |||
ADAT1 Antibody (C-5) AC | sc-271812 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ADAT1 Antibody (C-5) HRP | sc-271812 HRP | 200 µg/ml | $322.00 | |||
ADAT1 Antibody (C-5) FITC | sc-271812 FITC | 200 µg/ml | $336.00 | |||
ADAT1 Antibody (C-5) PE | sc-271812 PE | 200 µg/ml | $349.00 | |||
ADAT1 Antibody (C-5) Alexa Fluor® 488 | sc-271812 AF488 | 200 µg/ml | $364.00 | |||
ADAT1 Antibody (C-5) Alexa Fluor® 546 | sc-271812 AF546 | 200 µg/ml | $364.00 | |||
ADAT1 Antibody (C-5) Alexa Fluor® 594 | sc-271812 AF594 | 200 µg/ml | $364.00 | |||
ADAT1 Antibody (C-5) Alexa Fluor® 647 | sc-271812 AF647 | 200 µg/ml | $364.00 | |||
ADAT1 Antibody (C-5) Alexa Fluor® 680 | sc-271812 AF680 | 200 µg/ml | $364.00 | |||
ADAT1 Antibody (C-5) Alexa Fluor® 790 | sc-271812 AF790 | 200 µg/ml | $364.00 | |||
ADAT1 (C-5) Neutralizing Peptide | sc-271812 P | 100 µg/0.5 ml | $69.00 |