ADAR2 Double Nickase Plasmid (h): sc-401166-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADAR2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ADAR2 Double Nickase Plasmid (h) and ADAR2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADARB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ADAR2 Antibody (C-6): sc-514581
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAR2 Double Nickase Plasmid (h) | sc-401166-NIC | 20 µg | $410.00 | |||
ADAR2 Double Nickase Plasmid (h2) | sc-401166-NIC-2 | 20 µg | $410.00 |
ADARB1 encodes ADAR2, an adenosine deaminase acting on RNA that catalyzes A-to-I editing within double-stranded RNA structures, thereby recoding transcripts and reshaping RNA secondary structure. ADAR2 activity is prominent in the nervous system and influences ion channel and neurotransmission-related mRNAs, linking RNA editing to excitability, synaptic signaling, and neuronal development. Beyond recoding, ADAR2 can modulate RNA stability and splice site choice and helps tune innate immune sensing by altering dsRNA substrates that would otherwise engage pattern-recognition receptors. Dysregulated ADAR2-dependent editing patterns have been associated with neurological disease mechanisms and have been investigated in cancer and inflammation contexts where RNA editing rewires gene regulation.
ADAR2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADARB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADARB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADARB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADARB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.