ADAR2 Antibody (C-6): sc-514581
- ADAR2 Antibody (C-6) is a mouse monoclonal IgG2b κ ADAR2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 719-741 at the C-terminus of ADAR2 of human origin
- ADAR2 Antibody (C-6) is recommended for detection of ADAR2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ADAR2 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ADAR2 Antibody (C-6) for WB applications. These reagents are now offered in bundles with ADAR2 Antibody (C-6) (see ordering information below).
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ADAR2 Antibody (C-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects ADAR2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ADAR2 antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADAR2, also known as adenosine deaminase RNA-specific (RED1), plays a crucial role in RNA editing by converting adenosine to inosine in RNA molecules. This modification is vital for proper functioning of glutamate receptor subunit B, allowing generation of new splice sites and alteration of protein coding sequences, which significantly impact neuronal signaling and plasticity. ADAR2′s ability to modify its own pre-mRNA enhances editing efficiency, demonstrating a sophisticated regulatory mechanism essential for maintaining cellular homeostasis and response to environmental stimuli. Translocation of ADAR2 from nucleolus to nucleoplasm increases editing of substrates, highlighting dynamic nature of function within cells. Alternative splicing of ADAR2 results in several transcript variants, which may further influence RNA editing capabilities and functional diversity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ADAR2 Antibody (C-6) References:
- Double-stranded RNA adenosine deaminases ADAR1 and ADAR2 have overlapping specificities. | Lehmann, KA. and Bass, BL. 2000. Biochemistry. 39: 12875-84. PMID: 11041852
- Modulation of RNA editing by functional nucleolar sequestration of ADAR2. | Sansam, CL., et al. 2003. Proc Natl Acad Sci U S A. 100: 14018-23. PMID: 14612560
- Structure and sequence determinants required for the RNA editing of ADAR2 substrates. | Dawson, TR., et al. 2004. J Biol Chem. 279: 4941-51. PMID: 14660658
- Deficiency in RNA editing enzyme ADAR2 impairs regulated exocytosis. | Yang, L., et al. 2010. FASEB J. 24: 3720-32. PMID: 20501795
- Pin1 and WWP2 regulate GluR2 Q/R site RNA editing by ADAR2 with opposing effects. | Marcucci, R., et al. 2011. EMBO J. 30: 4211-22. PMID: 21847096
- ADAR2 induces reproducible changes in sequence and abundance of mature microRNAs in the mouse brain. | Vesely, C., et al. 2014. Nucleic Acids Res. 42: 12155-68. PMID: 25260591
- Probing RNA recognition by human ADAR2 using a high-throughput mutagenesis method. | Wang, Y. and Beal, PA. 2016. Nucleic Acids Res. 44: 9872-9880. PMID: 27614075
- RNA editing enzyme ADAR2 is a mediator of neuropathic pain after peripheral nerve injury. | Uchida, H., et al. 2017. FASEB J. 31: 1847-1855. PMID: 28126736
- A-to-I RNA editing enzyme ADAR2 regulates light-induced circadian phase-shift. | Terajima, H., et al. 2018. Sci Rep. 8: 14848. PMID: 30287844
- A new strategy to increase RNA editing at the Q/R site of GluA2 AMPA receptor subunits by targeting alternative splicing patterns of ADAR2. | Chaytow, H., et al. 2021. J Neurosci Methods. 364: 109357. PMID: 34536489
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAR2 Antibody (C-6) | sc-514581 | 200 µg/ml | $322.00 | |||
ADAR2 Antibody (C-6): m-IgGκ BP-HRP Bundle | sc-524780 | 200 µg Ab, 40 µg BP | $361.00 | |||
ADAR2 Antibody (C-6): m-IgG2b BP-HRP Bundle | sc-549183 | 200 µg Ab; 10 µg BP | $361.00 | |||
ADAR2 Antibody (C-6) AC | sc-514581 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ADAR2 Antibody (C-6) HRP | sc-514581 HRP | 200 µg/ml | $322.00 | |||
ADAR2 Antibody (C-6) FITC | sc-514581 FITC | 200 µg/ml | $336.00 | |||
ADAR2 Antibody (C-6) PE | sc-514581 PE | 200 µg/ml | $349.00 | |||
ADAR2 Antibody (C-6) Alexa Fluor® 488 | sc-514581 AF488 | 200 µg/ml | $364.00 | |||
ADAR2 Antibody (C-6) Alexa Fluor® 546 | sc-514581 AF546 | 200 µg/ml | $364.00 | |||
ADAR2 Antibody (C-6) Alexa Fluor® 594 | sc-514581 AF594 | 200 µg/ml | $364.00 | |||
ADAR2 Antibody (C-6) Alexa Fluor® 647 | sc-514581 AF647 | 200 µg/ml | $364.00 | |||
ADAR2 Antibody (C-6) Alexa Fluor® 680 | sc-514581 AF680 | 200 µg/ml | $364.00 | |||
ADAR2 Antibody (C-6) Alexa Fluor® 790 | sc-514581 AF790 | 200 µg/ml | $364.00 | |||
ADAR2 (C-6) Neutralizing Peptide | sc-514581 P | 100 µg/0.5 ml | $69.00 |