Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ADAM9 CRISPR/Cas9 KO Plasmid (m): sc-418994
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADAM9 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ADAM9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ADAM9 Antibody (G-1): sc-377233
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAM9 CRISPR/Cas9 KO Plasmid (m) | sc-418994 | 20 µg | $397.00 |
Overview
Adam9 encodes ADAM9, a membrane-anchored metalloprotease-disintegrin that mediates ectodomain shedding of cell-surface proteins and contributes to extracellular matrix remodeling. Through proteolytic processing of receptors, adhesion molecules, and growth factor precursors, ADAM9 modulates signaling networks such as EGFR/ErbB-related pathways, integrin-dependent adhesion, and migratory responses. In mouse systems, ADAM9 has been linked to regulation of inflammation, tissue injury responses, and tumor-associated microenvironmental processes, making it relevant to studies of fibrosis, neuroinflammation, and cancer biology. Its activity influences cell–cell communication and protease cascades that shape epithelial–stromal interactions and immune cell trafficking.
ADAM9 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adam9 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adam9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adam9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ADAM9 protein expression.
This CRISPR knockout system enables efficient generation of Adam9-deficient cell models for investigation of ADAM9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adam9 exon(s) critical for ADAM9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adam9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ADAM9 CRISPR/Cas9 KO Plasmid (m) and ADAM9 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adam9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ADAM9 HDR Plasmid (m) and ADAM9 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adam9 homology arms to support homology-directed repair at defined Adam9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.