ADAM8 CRISPR/Cas9 KO Plasmid (m): sc-418993
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADAM8 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ADAM8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAM8 CRISPR/Cas9 KO Plasmid (m) | sc-418993 | 20 µg | $397.00 |
Overview
Adam8 encodes a transmembrane metalloprotease-disintegrin (ADAM8) that mediates ectodomain shedding and cell–cell or cell–matrix interactions, integrating proteolysis with adhesion signaling. In mouse immune and stromal compartments, ADAM8 contributes to inflammatory signaling, leukocyte recruitment, and tissue remodeling through regulation of surface receptors and cytokine/chemokine availability. Its activity intersects with pathways linked to MAPK and NF-κB signaling outputs by modulating receptor activation and extracellular microenvironment cues. Dysregulated Adam8 expression has been associated with chronic inflammation, fibrosis, and tumor-associated processes such as invasion and metastatic niche remodeling, making it relevant for mechanistic studies in disease models.
ADAM8 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adam8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adam8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adam8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ADAM8 protein expression.
This CRISPR knockout system enables efficient generation of Adam8-deficient cell models for investigation of ADAM8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adam8 exon(s) critical for ADAM8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adam8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ADAM8 CRISPR/Cas9 KO Plasmid (m) and ADAM8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adam8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ADAM8 HDR Plasmid (m) and ADAM8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adam8 homology arms to support homology-directed repair at defined Adam8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.