ADAM7 CRISPR/Cas9 KO Plasmid (m2): sc-418992-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADAM7 CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ADAM7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAM7 CRISPR/Cas9 KO Plasmid (m2) | sc-418992-KO-2 | 20 µg | $397.00 |
Overview
Adam7 encodes ADAM7, a catalytically inactive member of the ADAM (a disintegrin and metalloprotease) family that functions as a membrane-associated adhesion and signaling modulator rather than a protease. In mouse, ADAM7 is enriched in the male reproductive tract and participates in sperm maturation and sperm–egg interactions through protein–protein interactions and coordination of cell surface receptor complexes. It contributes to membrane organization and cell–cell communication processes that intersect with pathways governing extracellular matrix interactions and cellular adhesion dynamics. Dysregulation of ADAM family members is broadly linked to altered tissue remodeling and inflammatory signaling, making Adam7 a useful locus for mechanistic studies of reproductive biology and cell-surface signaling networks.
ADAM7 CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Adam7 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adam7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adam7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ADAM7 protein expression.
This CRISPR knockout system enables efficient generation of Adam7-deficient cell models for investigation of ADAM7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adam7 exon(s) critical for ADAM7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adam7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ADAM7 CRISPR/Cas9 KO Plasmid (m) and ADAM7 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adam7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ADAM7 HDR Plasmid (m) and ADAM7 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adam7 homology arms to support homology-directed repair at defined Adam7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.