Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ADAM15 CRISPR/Cas9 KO Plasmid (h): sc-405627
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADAM15 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ADAM15 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ADAM15 Antibody (D-5): sc-365752
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAM15 CRISPR/Cas9 KO Plasmid (h) | sc-405627 | 20 µg | $397.00 |
Overview
ADAM15 encodes a transmembrane metalloprotease-disintegrin that participates in ectodomain shedding and cell–cell/cell–matrix interactions through its protease, disintegrin, and cytoplasmic domains. It modulates signaling networks linked to adhesion and motility, including integrin-associated pathways, and can influence availability of membrane-tethered ligands and receptors via regulated proteolysis. ADAM15 activity has been connected to processes such as migration, invasion, and inflammatory signaling, and altered expression has been reported across multiple disease contexts including cancer progression and vascular pathology. As a surface-associated protease, ADAM15 is frequently studied for its contributions to extracellular remodeling and signal transduction crosstalk at the plasma membrane.
ADAM15 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ADAM15 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ADAM15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ADAM15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ADAM15 protein expression.
This CRISPR knockout system enables efficient generation of ADAM15-deficient cell models for investigation of ADAM15 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ADAM15 exon(s) critical for ADAM15 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ADAM15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ADAM15 CRISPR/Cas9 KO Plasmid (h) and ADAM15 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ADAM15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ADAM15 HDR Plasmid (h) and ADAM15 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ADAM15 homology arms to support homology-directed repair at defined ADAM15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.