ADAM11 CRISPR/Cas9 KO Plasmid (m): sc-418982
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADAM11 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ADAM11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAM11 CRISPR/Cas9 KO Plasmid (m) | sc-418982 | 20 µg | $397.00 |
Overview
Adam11 encodes ADAM11, a catalytically inactive ADAM family disintegrin-like protein implicated in cell–cell and cell–matrix interactions, with prominent roles in the nervous system. ADAM11 is associated with processes such as neuronal differentiation, synapse organization, and modulation of adhesion-dependent signaling through its extracellular domains. Altered ADAM11 expression and genomic changes have been reported in studies of neurodevelopmental and neurodegenerative phenotypes, and it is also investigated in the context of tumor biology where adhesion and migratory programs are dysregulated. As a mouse gene, Adam11 provides a tractable system for dissecting conserved ADAM-mediated mechanisms in neural circuitry and tissue remodeling.
ADAM11 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adam11 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adam11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adam11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ADAM11 protein expression.
This CRISPR knockout system enables efficient generation of Adam11-deficient cell models for investigation of ADAM11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adam11 exon(s) critical for ADAM11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adam11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ADAM11 CRISPR/Cas9 KO Plasmid (m) and ADAM11 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adam11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ADAM11 HDR Plasmid (m) and ADAM11 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adam11 homology arms to support homology-directed repair at defined Adam11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.