Ada Antibody (ADA-1): sc-53152

Ada Antibody (ADA-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Ada protein of E. coli origin by western blotting (WB) and immunoprecipitation (IP). Anti-Ada antibody (ADA-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ada protein plays a crucial role in the repair of alkylated guanine in DNA, which is vital for maintaining genomic integrity and preventing mutations that could lead to cell malfunction or disease. Ada protein is a monomeric, globular structure composed of 353 amino acids and features two distinct alkylacceptor domains that facilitate repair functions. Specifically, Ada protein can accept alkyl groups from the O-6 position of alkylguanine in DNA at cysteine residue 321 and from alkyl phosphotriester at cysteine residue 69, effectively inactivating the enzyme in the process. Ada protein is also capable of repairing O-4-methylthymine, showcasing versatility in DNA repair mechanisms. The N-terminal region of Ada protein is essential for the activation of transcription of genes such as ALKA, ALKB, and AIDB, highlighting regulatory role in cellular responses to DNA damage.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ada Antibody (ADA-1) References:

1. DNA-binding mechanism of the Escherichia coli Ada O(6)-alkylguanine-DNA alkyltransferase.  |  Verdemato, PE., et al. 2000. Nucleic Acids Res. 28: 3710-8. PMID: 11000262
2. Molecular mechanisms of adaptive response to alkylating agents in Escherichia coli and some remarks on O(6)-methylguanine DNA-methyltransferase in other organisms.  |  Kleibl, K. 2002. Mutat Res. 512: 67-84. PMID: 12220590
3. Recent progress on the Ada response for inducible repair of DNA alkylation damage.  |  Sedgwick, B. and Lindahl, T. 2002. Oncogene. 21: 8886-94. PMID: 12483506
4. 1H, 13C and 15N resonance assignments of the N-terminal 16 kDa domain of Escherichia coli Ada protein.  |  Takinowaki, H., et al. 2004. J Biomol NMR. 29: 447-8. PMID: 15213462
5. Adaptive response to alkylating agents involves alteration in situ of O6-methylguanine residues in DNA.  |  Karran, P., et al. 1979. Nature. 280: 76-7. PMID: 15305585
6. [O6-benzylguanine stimulates regulatory functions of the Ada protein in Escherichia coli].  |  Vasil'eva, SV., et al. 2005. Genetika. 41: 1462-6. PMID: 16358713
7. The solution structure of the methylated form of the N-terminal 16-kDa domain of Escherichia coli Ada protein.  |  Takinowaki, H., et al. 2006. Protein Sci. 15: 487-97. PMID: 16452614
8. Mutations in the Ada O6-alkylguanine-DNA alkyltransferase conferring sensitivity to inactivation by O6-benzylguanine and 2,4-diamino-6-benzyloxy-5-nitrosopyrimidine.  |  Crone, TM., et al. 1995. Carcinogenesis. 16: 1687-92. PMID: 7634390
9. Reversible folding of Ada protein (O6-methylguanine-DNA methyltransferase) of Escherichia coli.  |  Bhattacharyya, D., et al. 1998. Biochemistry. 37: 1722-30. PMID: 9484244

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ada Antibody (ADA-1) has 1 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ada Antibody (ADA-1) Specifications

Alternate Product Name: Ada

Product Clone Name: ADA-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Ada

Epitope Species: E. coli

Product Reactivity/Detection: Ada

Species Reactivity/Detection: E. coli

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ada Antibody (ADA-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin