Actin Antibody (NH3): sc-58679
- Actin Antibody (NH3) is a mouse monoclonal IgM κ, cited in 17 publications, provided at 200 µg/ml
- raised against monocytes and U-937 histolytic lymphoma of human origin
- recommended for detection of Actin of mouse, rat, human and rabbit origin by WB, IP, IF, IHC(P) and FCM; non cross-reactive with the N-terminal 40 amino acids
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See beta Actin (C4): sc-47778 for Actin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Actin Antibody (NH3). This work is in progress.
QUICK LINKS
SEE ALSO...
Actin Antibody (NH3) is a mouse monoclonal IgM kappa light chain antibody designed for species reactivity in mouse, rat, human, and rabbit, with its epitope raised against monocytes and U-937 histiocytic lymphoma of human origin. Actin, one of the most abundant and essential proteins in eukaryotic cells, plays a pivotal role in maintaining cell structure and function. Actin forms dynamic filamentous structures (F-actin) involved in various critical cellular processes such as intracellular transport, adhesion, migration, cytokinesis, and cell shape regulation. In higher eukaryotes, multiple isoforms of actin are expressed, each tailored to specific functions: α-actin is predominantly found in muscle tissues, whereas β- and γ-actin are more commonly associated with non-muscle cells. This diversity in isoforms enables actin to fulfill roles ranging from providing structural support to regulating gene expression and controlling cell signaling pathways. In immune cells like monocytes, actin plays a vital role in processes like phagocytosis and immune response. U-937 cells, derived from human histiocytic lymphoma, are often used in research to study immune-related functions, particularly phagocyte differentiation. Understanding actin′s role in these cells can offer insights into diseases like cancer and immune disorders, making anti-actin antibody (NH3) a critical tool in research. Additionally, actin dynamics are regulated by various actin-binding proteins (ABPs), which influence processes like cell motility and contraction, further emphasizing actin′s significance in cellular functions. By using techniques like western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM), researchers can study cell structure, motility, and various cellular processes that rely on actin dynamics.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Actin Antibody (NH3) | sc-58679 | 200 µg/ml | $316.00 | |||
Actin Antibody (NH3) FITC | sc-58679 FITC | 200 µg/ml | $330.00 | |||
Actin Antibody (NH3) PE | sc-58679 PE | 200 µg/ml | $343.00 |