Actin Antibody (8.S2.1): sc-70319

Actin Antibody (8.S2.1) is a mouse monoclonal IgM kappa light chain antibody raised against monocytes and U-937 histolytic lymphoma of human origin, demonstrating reactivity with mouse, rat, human, and rabbit species. Actin plays a critical role in forming microfilaments within the cytoskeleton, existing in both globular (G-actin) and filamentous (F-actin) forms, which are essential for various cellular processes such as muscle contraction, cell motility, and maintenance of cell shape and integrity. Actin filaments can form both stable structures, like those in muscle sarcomeres, and dynamic structures, such as the leading edge of motile cells. While primarily localized in the cytoplasm, actin is also present in the nucleus, contributing to chromatin remodeling and gene transcription. The dynamics of actin are regulated by small GTPases like Rho, Rac, and Cdc42: Rho regulates the formation of stress fibers and focal adhesions; Rac controls actin accumulation at the plasma membrane leading to lamellipodia formation; and Cdc42 stimulates the formation of filopodia. Actin interacts with numerous proteins, including myosin for muscle contraction, tropomyosin and troponin for muscle regulation, and proteins like profilin and cofilin that regulate actin polymerization and depolymerization. These interactions are crucial for cell movement, division, and intracellular transport, underscoring the importance of actin in maintaining cellular function and structure. Anti-actin antibody (8.S2.1) is a valuable tool for researchers studying actin dynamics and related protein-protein interactions across various species.

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Actin Antibody (8.S2.1) References:

1. Repolymerization of actin from actin:thymosin beta4 complex induced by diaphanous related formins and gelsolin.  |  Mannherz, HG., et al. 2010. Ann N Y Acad Sci. 1194: 36-43. PMID: 20536448
2. ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization.  |  Heisler, DB., et al. 2015. Science. 349: 535-9. PMID: 26228148
3. Actin, actin-binding proteins, and actin-related proteins in the nucleus.  |  Kristó, I., et al. 2016. Histochem Cell Biol. 145: 373-88. PMID: 26847179
4. Nuclear Actin and Actin-Binding Proteins in DNA Repair.  |  Hurst, V., et al. 2019. Trends Cell Biol. 29: 462-476. PMID: 30954333
5. Involvement of Actin and Actin-Binding Proteins in Carcinogenesis.  |  Izdebska, M., et al. 2020. Cells. 9: PMID: 33036298
6. Mechanically tuning actin filaments to modulate the action of actin-binding proteins.  |  Jégou, A. and Romet-Lemonne, G. 2021. Curr Opin Cell Biol. 68: 72-80. PMID: 33160108
7. Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells.  |  Jung, M., et al. 2020. Front Cell Dev Biol. 8: 588556. PMID: 33324645
8. Actin-binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin II.  |  Hayakawa, Y., et al. 2023. Life Sci Alliance. 6: PMID: 36288901
9. Septins mediate a microtubule-actin crosstalk that enables actin growth on microtubules.  |  Nakos, K., et al. 2022. Proc Natl Acad Sci U S A. 119: e2202803119. PMID: 36475946
10. LRRK2 decreases microglial actin dynamics by filamentous actin depolymerization and Rac1 inhibition.  |  Kim, B., et al. 2022. Anim Cells Syst (Seoul). 26: 380-387. PMID: 36605588

The preferred beta Actin antibody is beta Actin Antibody (C4): sc-47778.