ACSVL1 Double Nickase Plasmid (h): sc-405122-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACSVL1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACSVL1 Double Nickase Plasmid (h) and ACSVL1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC27A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACSVL1 Antibody (D-7): sc-393906
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACSVL1 Double Nickase Plasmid (h) | sc-405122-NIC | 20 µg | $410.00 | |||
ACSVL1 Double Nickase Plasmid (h2) | sc-405122-NIC-2 | 20 µg | $410.00 |
SLC27A2 encodes acyl-CoA synthetase very long-chain 1 (ACSVL1/FATP2), a membrane-associated enzyme that activates long-chain and very-long-chain fatty acids to acyl-CoA thioesters, enabling their channeling into β-oxidation, complex lipid synthesis, and lipid remodeling. By controlling fatty acid uptake and intracellular activation, ACSVL1 influences mitochondrial and peroxisomal lipid metabolism, energy homeostasis, and cellular responses to nutrient availability. Dysregulated fatty acid handling involving SLC27A2 has been studied in metabolic phenotypes such as hepatic steatosis and insulin resistance, and altered lipid metabolic programs relevant to inflammation and tumor cell bioenergetics. These properties make ACSVL1 a useful node for dissecting lipid-driven signaling and metabolic reprogramming in human cell models.
ACSVL1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC27A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC27A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC27A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC27A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.