ACSL4 Double Nickase Plasmid (m): sc-424503-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACSL4 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACSL4 Double Nickase Plasmid (m) and ACSL4 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Acsl4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACSL4 Antibody (F-4): sc-365230
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACSL4 Double Nickase Plasmid (m) | sc-424503-NIC | 20 µg | $410.00 | |||
ACSL4 Double Nickase Plasmid (m2) | sc-424503-NIC-2 | 20 µg | $410.00 |
Mouse Acsl4 encodes acyl-CoA synthetase long-chain family member 4 (ACSL4), an enzyme that converts polyunsaturated long-chain fatty acids such as arachidonic acid and adrenic acid into their acyl-CoA derivatives for incorporation into membrane phospholipids. By shaping phospholipid remodeling and lipid mediator availability, ACSL4 influences ferroptosis sensitivity, oxidative lipid metabolism, and membrane dynamics linked to cellular stress responses. ACSL4 activity intersects with pathways regulating lipid peroxidation and inflammatory signaling, and altered expression or function has been associated with neurodevelopmental phenotypes, metabolic dysregulation, and context-dependent changes in tumor biology. These properties make Acsl4 a useful target for interrogating lipid-driven mechanisms in cell death, redox homeostasis, and membrane composition in mouse model systems.
ACSL4 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Acsl4 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Acsl4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Acsl4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Acsl4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.