ACSL3 Double Nickase Plasmid (h): sc-403546-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACSL3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACSL3 Double Nickase Plasmid (h) and ACSL3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACSL3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACSL3 Antibody (H-9): sc-166374
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACSL3 Double Nickase Plasmid (h) | sc-403546-NIC | 20 µg | $410.00 | |||
ACSL3 Double Nickase Plasmid (h2) | sc-403546-NIC-2 | 20 µg | $410.00 |
ACSL3 (acyl-CoA synthetase long-chain family member 3) is an endoplasmic reticulum–associated enzyme that activates long-chain fatty acids to acyl-CoA thioesters, a required step for lipid biosynthesis and fatty acid utilization. By channeling fatty acids into phospholipid and neutral lipid production, ACSL3 influences membrane remodeling, lipid droplet biogenesis, and broader metabolic programs linked to cellular growth and stress adaptation. Its activity interfaces with pathways governing fatty acid uptake, ER lipid homeostasis, and mitochondrial lipid supply, shaping bioenergetics and signaling lipid availability. Altered ACSL3 regulation has been investigated in contexts of metabolic rewiring, lipid-associated inflammation, and oncogenic transformation where fatty acid activation can contribute to phenotype-defining lipid profiles.
ACSL3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACSL3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACSL3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACSL3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACSL3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.