Acrosin CRISPR/Cas9 KO Plasmid (h): sc-402292
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Acrosin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Acrosin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Acrosin CRISPR/Cas9 KO Plasmid (h) | sc-402292 | 20 µg | $397.00 |
Overview
ACR encodes acrosin, a serine protease synthesized as the zymogen proacrosin and stored in the acrosome of human spermatozoa. Upon capacitation and the acrosome reaction, acrosin participates in regulated proteolysis and substrate binding that support sperm interaction with the zona pellucida and progression through extracellular matrices during fertilization. Its activity links to acrosomal exocytosis, protease activation cascades, and remodeling of sperm surface proteins during gamete recognition. Altered ACR expression or acrosin function is studied in the context of male reproductive biology and infertility-associated phenotypes, including impaired sperm–zona binding and reduced fertilization competence.
Acrosin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ACR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ACR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ACR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Acrosin protein expression.
This CRISPR knockout system enables efficient generation of ACR-deficient cell models for investigation of Acrosin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ACR exon(s) critical for Acrosin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ACR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Acrosin CRISPR/Cas9 KO Plasmid (h) and Acrosin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ACR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Acrosin HDR Plasmid (h) and Acrosin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ACR homology arms to support homology-directed repair at defined ACR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.