AChR alpha 8 Antibody (309) recommended for detection of nicotinic AChRα8 of avian origin by WB and IF

AChRα8 Antibody (309): sc-65868

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Datasheets

    • Anti-AChRα8 Antibody (309) is a rat monoclonal IgG1 AChRα8 antibody provided at 200 µg/ml
    • raised against bacterially expressed large cytoplasmic domain fragment of chicken origin
    • recommended for detection of nicotinic AChRα8 of avian origin by WB and IF

How much protein do you recommend loading on the gel for Western blot detection using AChRα8 (309): sc-65868 monoclonal antibody?

Asked by: AbPolly
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-05
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