Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody (207): sc-65838
- Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody (207) is a rat monoclonal IgG2a provided at 200 µg/ml
- raised against purifed muscle AChR of human origin
- recommended for detection of nicotinic AChRα1 of human origin by WB and IF
- See Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 (153): sc-65829 for Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody (207). This work is in progress.
AChRα1 Antibody (207) is a rat monoclonal IgG2a antibody that detects AChRα1 in human samples through applications such as western blotting (WB) and immunofluorescence (IF). AChRα1, also known as ACHRD, CHRNA, CMS2A, FCCMS, SCCMS, or CHRNA1, is a crucial component of the nicotinic acetylcholine receptor family, which plays a vital role in neurotransmission at the neuromuscular junction. This multi-pass membrane protein consists of 482 amino acids and exists in two alternatively spliced isoforms, with isoform 1 being specifically expressed in skeletal muscle and isoform 2 found in a variety of tissues, including skeletal muscle, brain, heart, kidney, liver, lung, and thymus. The presence of AChRα1 at the postsynaptic membrane allows for the binding of acetylcholine, facilitating the movement of ions through the receptor′s transmembrane aqueous pore, which is essential for muscle contraction and signal transmission in the nervous system. The activity of AChRα1 is tightly regulated, as the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine, ensuring precise control over synaptic transmission. The ability of AChRα1 to undergo post-translational modifications, such as glycosylation, is also significant, as these modifications can influence receptor function, stability, and interactions with other proteins, ultimately impacting neuromuscular signaling and muscle function. Anti-AChRα1 antibody (207) is an invaluable tool for researchers studying the dynamics of neurotransmission and the molecular mechanisms underlying neuromuscular disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody (207) References:
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- Structural determinants for alpha-neurotoxin sensitivity in muscle nAChR and their implications for the gating mechanism. | Dellisanti, CD., et al. 2007. Channels (Austin). 1: 234-7. PMID: 18708746
- HnRNP L and hnRNP LL antagonistically modulate PTB-mediated splicing suppression of CHRNA1 pre-mRNA. | Rahman, MA., et al. 2013. Sci Rep. 3: 2931. PMID: 24121633
- Identifying barbiturate binding sites in a nicotinic acetylcholine receptor with [3H]allyl m-trifluoromethyldiazirine mephobarbital, a photoreactive barbiturate. | Hamouda, AK., et al. 2014. Mol Pharmacol. 85: 735-46. PMID: 24563544
- Subunit interfaces contribute differently to activation and allosteric modulation of neuronal nicotinic acetylcholine receptors. | Short, CA., et al. 2015. Neuropharmacology. 91: 157-68. PMID: 25486620
- Neuromuscular junctions (NMJs): ultrastructural analysis and nicotinic acetylcholine receptor (nAChR) subunit mRNA expression in offspring subjected to protein restriction throughout pregnancy. | Castro, PATS., et al. 2017. Int J Exp Pathol. 98: 109-116. PMID: 28543723
- Enantiomeric barbiturates bind distinct inter- and intrasubunit binding sites in a nicotinic acetylcholine receptor (nAChR). | Yu, Z. and Cohen, JB. 2017. J Biol Chem. 292: 17258-17271. PMID: 28878016
- Blocking skeletal muscle DHPRs/Ryr1 prevents neuromuscular synapse loss in mutant mice deficient in type III Neuregulin 1 (CRD-Nrg1). | Liu, Y., et al. 2019. PLoS Genet. 15: e1007857. PMID: 30870432
- Effects of DDT and DDE on placental cholinergic receptors. | Uyar, R., et al. 2024. Reprod Toxicol. 126: 108588. PMID: 38615785
- Cytochalasin modulation of nicotinic cholinergic receptor expression and muscarinic receptor function in human TE671/RD cells: a possible functional role of the cytoskeleton. | Bencherif, M. and Lukas, RJ. 1993. J Neurochem. 61: 852-64. PMID: 8360687
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody (207) | sc-65838 | 200 µg/ml | $322.00 |