ACBD6 CRISPR/Cas9 KO Plasmid (m): sc-428356
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACBD6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACBD6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACBD6 CRISPR/Cas9 KO Plasmid (m) | sc-428356 | 20 µg | $397.00 |
Overview
Acbd6 encodes acyl-CoA binding domain containing 6 (ACBD6), a cytosolic lipid-binding protein that associates with long-chain acyl-CoA esters and helps coordinate intracellular acyl-CoA handling. By buffering and presenting activated fatty acids to downstream enzymes, ACBD6 is positioned to influence lipid metabolic flux, membrane lipid remodeling, and acylation-dependent processes that couple nutrient state to cellular function. Perturbation of acyl-CoA availability can impact mitochondrial metabolism, oxidative stress responses, and inflammatory signaling, making Acbd6 relevant to studies of metabolic dysregulation and lipid-driven cellular phenotypes. Mouse ACBD6 is therefore a useful entry point for dissecting acyl-CoA–regulated pathways in tissues with high lipid turnover, including liver, adipose, and immune cells.
ACBD6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Acbd6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Acbd6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Acbd6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACBD6 protein expression.
This CRISPR knockout system enables efficient generation of Acbd6-deficient cell models for investigation of ACBD6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Acbd6 exon(s) critical for ACBD6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Acbd6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACBD6 CRISPR/Cas9 KO Plasmid (m) and ACBD6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Acbd6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACBD6 HDR Plasmid (m) and ACBD6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Acbd6 homology arms to support homology-directed repair at defined Acbd6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.