ACBD5 CRISPR/Cas9 KO Plasmid (h): sc-417183
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACBD5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACBD5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACBD5 CRISPR/Cas9 KO Plasmid (h) | sc-417183 | 20 µg | $397.00 |
Overview
ACBD5 encodes an acyl-CoA binding domain–containing peroxisomal protein that supports intracellular lipid handling by binding long-chain acyl-CoA esters and coordinating peroxisome-associated lipid metabolism. It localizes to the peroxisome membrane and contributes to peroxisome–organelle communication, influencing fatty acid trafficking and the organization of peroxisomal pathways involved in lipid homeostasis. Through its role in peroxisomal dynamics and lipid processing, ACBD5 is relevant to cellular stress responses linked to altered lipid composition and organelle dysfunction. Dysregulation of peroxisome-dependent lipid metabolism is frequently studied in the context of neurodegeneration, metabolic imbalance, and inflammatory signaling, making ACBD5 a useful target for mechanistic investigations.
ACBD5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ACBD5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ACBD5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ACBD5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACBD5 protein expression.
This CRISPR knockout system enables efficient generation of ACBD5-deficient cell models for investigation of ACBD5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ACBD5 exon(s) critical for ACBD5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ACBD5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACBD5 CRISPR/Cas9 KO Plasmid (h) and ACBD5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ACBD5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACBD5 HDR Plasmid (h) and ACBD5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ACBD5 homology arms to support homology-directed repair at defined ACBD5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.