ACBD3 Antibody (518): sc-101277

ACBD3 Antibody (518) is a mouse monoclonal IgG1 kappa light chain antibody that detects ACBD3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-ACBD3 antibody (518) is available as a non-conjugated format. ACBD3 protein, also known as acyl-CoA-binding domain-containing protein 3, GCP60, GOCAP1, PAP7, or GOLPH1, is a critical Golgi apparatus membrane protein characterized by the presence of one acyl-CoA-binding domain and one GOLD domain, which are essential for protein interactions. ACBD3 is expressed ubiquitously, with highest levels found in ovary and testis, and plays a vital role in maintaining Golgi structure. ACBD3 interacts with Giantin, a member of the golgin subfamily, to facilitate protein transport between Golgi and endoplasmic reticulum, thereby influencing cellular processes. Proper localization and function of ACBD3 are crucial, as alterations in subcellular distribution can initiate signaling cascades within Golgi that regulate cell fate and cycle progression. ACBD3 functions as a peripheral-type benzodiazepine receptor-associated protein, potentially impacting hormonal regulation and steroidogenesis, making ACBD3 an important target for research in cellular signaling and metabolism.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ACBD3 Antibody (518) References:

1. Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin.  |  Sohda, M., et al. 2001. J Biol Chem. 276: 45298-306. PMID: 11590181
2. Identification, localization, and function in steroidogenesis of PAP7: a peripheral-type benzodiazepine receptor- and PKA (RIalpha)-associated protein.  |  Li, H., et al. 2001. Mol Endocrinol. 15: 2211-28. PMID: 11731621
3. Molecular cloning, genomic organization, chromosomal mapping and subcellular localization of mouse PAP7: a PBR and PKA-RIalpha associated protein.  |  Liu, J., et al. 2003. Gene. 308: 1-10. PMID: 12711385
4. GCP60 preferentially interacts with a caspase-generated golgin-160 fragment.  |  Sbodio, JI., et al. 2006. J Biol Chem. 281: 27924-31. PMID: 16870622
5. NMDA receptor-nitric oxide transmission mediates neuronal iron homeostasis via the GTPase Dexras1.  |  Cheah, JH., et al. 2006. Neuron. 51: 431-40. PMID: 16908409
6. The mammalian Golgi regulates numb signaling in asymmetric cell division by releasing ACBD3 during mitosis.  |  Zhou, Y., et al. 2007. Cell. 129: 163-78. PMID: 17418793
7. Identification of a redox-sensitive cysteine in GCP60 that regulates its interaction with golgin-160.  |  Sbodio, JI. and Machamer, CE. 2007. J Biol Chem. 282: 29874-81. PMID: 17711851
8. ACBD3 is required for FAPP2 transferring glucosylceramide through maintaining the Golgi integrity.  |  Liao, J., et al. 2019. J Mol Cell Biol. 11: 107-117. PMID: 29750412
9. Acyl-CoA-Binding Domain-Containing 3 (ACBD3; PAP7; GCP60): A Multi-Functional Membrane Domain Organizer.  |  Yue, X., et al. 2019. Int J Mol Sci. 20: PMID: 31022988
10. ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.  |  Yue, X., et al. 2021. BMC Biol. 19: 194. PMID: 34493279
11. ACBD3 Bioinformatic Analysis and Protein Expression in Breast Cancer Cells.  |  Houghton-Gisby, J., et al. 2022. Int J Mol Sci. 23: PMID: 36012147
12. An A-kinase anchoring protein (ACBD3) coordinates traffic-induced PKA activation at the Golgi.  |  Jia, J., et al. 2023. J Biol Chem. 299: 104696. PMID: 37044218

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACBD3 Antibody (518) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-ACBD3 Antibody (518) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACBD3 Antibody (518) Specifications

Alternate Product Name: ACBD3

Product Clone Name: 518

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 73-172

Epitope Target: ACBD3

Epitope Species: human

Product Reactivity/Detection: ACBD3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for ACBD3 Antibody (518): N/A