Abi-2 Antibody (B-3): sc-393982
- Abi-2 Antibody (B-3) is a mouse monoclonal IgG1 κ Abi-2 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 220-249 within an internal region of Abi-2 of human origin
- Abi-2 Antibody (B-3) is recommended for detection of Abi-2 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-Abi-2 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Abi-2 Antibody (B-3) for WB applications. These reagents are now offered in bundles with Abi-2 Antibody (B-3) (see ordering information below).
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Abi-2 Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Abi-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Abi-2 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Abi-2 protein plays a crucial role in cellular signaling pathways, particularly in the context of the Abelson oncogene, which was first identified as a viral transforming agent in Abelson murine leukemia virus. The Abelson gene (ABL1) encodes a tyrosine kinase essential for mitogenic signaling following growth factor receptor activation. Abi-2, along with Abi-1, contains SH3 domains that interact with proline-rich motifs of the Abl protein, enhancing kinase activity. This interaction regulates cell growth and transformation, as both Abi-1 and Abi-2 act as negative regulators in these processes. Abi proteins modulate actin polymerization in structures such as lamellipodia and filopodia, which are essential for cellular movement and tissue remodeling. Understanding Abi-2 function and interactions helps elucidate its role in cancer biology and potential therapeutic targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Abi-2 Antibody (B-3) References:
- Drosophila abelson interacting protein (dAbi) is a positive regulator of abelson tyrosine kinase activity. | Juang, JL. and Hoffmann, FM. 1999. Oncogene. 18: 5138-47. PMID: 10498863
- Abl interactor 1 binds to sos and inhibits epidermal growth factor- and v-Abl-induced activation of extracellular signal-regulated kinases. | Fan, PD. and Goff, SP. 2000. Mol Cell Biol. 20: 7591-601. PMID: 11003655
- The Abl interactor proteins localize to sites of actin polymerization at the tips of lamellipodia and filopodia. | Stradal, T., et al. 2001. Curr Biol. 11: 891-5. PMID: 11516653
- Inhibition of v-Abl transformation in 3T3 cells overexpressing different forms of the Abelson interactor protein Abi-1. | Ikeguchi, A., et al. 2001. Oncogene. 20: 4926-34. PMID: 11526477
- Three-dimensional solution structure of the src homology 2 domain of c-abl. | Overduin, M., et al. 1992. Cell. 70: 697-704. PMID: 1505033
- Lymphosarcoma: virus-induced thymic-independent disease in mice. | Abelson, HT. and Rabstein, LS. 1970. Cancer Res. 30: 2213-22. PMID: 4318922
- Sequences of the A-MuLV protein needed for fibroblast and lymphoid cell transformation. | Prywes, R., et al. 1983. Cell. 34: 569-79. PMID: 6193890
- Abl-interactor-1, a novel SH3 protein binding to the carboxy-terminal portion of the Abl protein, suppresses v-abl transforming activity. | Shi, Y., et al. 1995. Genes Dev. 9: 2583-97. PMID: 7590237
- ABI-1, a human homolog to mouse Abl-interactor 1, fuses the MLL gene in acute myeloid leukemia with t(10;11)(p11.2;q23). | Taki, T., et al. 1998. Blood. 92: 1125-30. PMID: 9694699
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Abi-2 Antibody (B-3) | sc-393982 | 200 µg/ml | $322.00 | |||
Abi-2 Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-527726 | 200 µg Ab; 10 µg BP | $361.00 | |||
Abi-2 Antibody (B-3): m-IgG1 BP-HRP Bundle | sc-533099 | 200 µg Ab; 20 µg BP | $361.00 | |||
Abi-2 Antibody (B-3) AC | sc-393982 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Abi-2 Antibody (B-3) HRP | sc-393982 HRP | 200 µg/ml | $322.00 | |||
Abi-2 Antibody (B-3) FITC | sc-393982 FITC | 200 µg/ml | $336.00 | |||
Abi-2 Antibody (B-3) PE | sc-393982 PE | 200 µg/ml | $349.00 | |||
Abi-2 Antibody (B-3) Alexa Fluor® 488 | sc-393982 AF488 | 200 µg/ml | $364.00 | |||
Abi-2 Antibody (B-3) Alexa Fluor® 546 | sc-393982 AF546 | 200 µg/ml | $364.00 | |||
Abi-2 Antibody (B-3) Alexa Fluor® 594 | sc-393982 AF594 | 200 µg/ml | $364.00 | |||
Abi-2 Antibody (B-3) Alexa Fluor® 647 | sc-393982 AF647 | 200 µg/ml | $364.00 | |||
Abi-2 Antibody (B-3) Alexa Fluor® 680 | sc-393982 AF680 | 200 µg/ml | $364.00 | |||
Abi-2 Antibody (B-3) Alexa Fluor® 790 | sc-393982 AF790 | 200 µg/ml | $364.00 | |||
Abi-2 (B-3) Neutralizing Peptide | sc-393982 P | 100 µg/0.5 ml | $69.00 |